BrdU incorporation reveals that the NB7-3 lineage generates three GMCs

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BrdU incorporation reveals that the NB7-3 lineage generates three GMCs BrdU incorporation reveals that the NB7-3 lineage generates three GMCs. Dorsal views; anterior is towards the left; the broken line indicates the midline; arrowheads, GW neuron. BrdU incorporation reveals that the NB7-3 lineage generates three GMCs. Dorsal views; anterior is towards the left; the broken line indicates the midline; arrowheads, GW neuron. (A-E) Labeling obtained in NB7-3-derived neurons at stage 15 after BrdU injections between 6.5 and 7.5 hours AEL. The corresponding images are shown underneath (A′-E′). Green, BrdU; red, Eg; yellow, doublestaining. We found all (A,A′), three (B,B′), two (C,C′), one (D,D′) and none (E,E′) of the neurons double labeled (yellow). The EW3 neuron is always labeled last (D,D′). GW and EW1 are always either both labeled together (A,B) or not labeled (C,D) indicating that they are siblings. (A′′-E′′) Diagram to explain how BrdU incorporation at different times during NB7-3 lineage development affects labelling. Vertical axis is the time axis; green, BrdU incorporation; green horizontal line, timepoint of BrdU application. (F,G) An Eg-positive NB7-3-derived cell cluster in a stage 14 Df(L3)H99 mutant embryo consisting of eight cells (G), compared with four in wild type (F). (H) In many hemisegments of CycA mutant embryos at stage 14, we find two cells in NB7-3 position where the most dorsal cell is smaller and Hb positive (yellow), indicating that this is the first born GMC. Eg is shown in red. Tanja Novotny et al. Development 2002;129:1027-1036 © 2002.