Synaptopodin expression is also in cells on Bowman’s capsule in juvenile human kidney. Synaptopodin expression is also in cells on Bowman’s capsule in juvenile human kidney. Staining of synaptopodin (green) and Hoechst (blue) on paraffin sections of kidneys derived from juvenile humans at different ages (A1 and A2, 2 weeks; B1 and B2, 5 months; C1–C3, 2 years; D1 and D2, 7 years of age). Arrowheads mark synaptopodin expression in cells on Bowman’s capsule. Visceral podocytes are densely packed on the glomerular tuft at younger ages (arrows). Scale bars, 50 µm. (E) Area covered by synaptopodin-positive cells on Bowman’s capsule in 20 random glomerular cross-sections for each time point shows a progressive decrease with increasing age. (F) Similarly, more area of Bowman’s (Bm’s) capsule was covered by synaptopodin-positive cells in smaller (and presumably, less mature) glomeruli. Tuft area was only measured in glomerular cross-sections, which also included the vascular stalk. Katja Berger et al. JASN 2014;25:693-705 ©2014 by American Society of Nephrology