Volume 10, Issue 5, Pages (May 2003)

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Volume 10, Issue 5, Pages 419-430 (May 2003) Characterization of the Mupirocin Biosynthesis Gene Cluster from Pseudomonas fluorescens NCIMB 10586 A.Kassem El-Sayed, Joanne Hothersall, Sian M. Cooper, Elton Stephens, Thomas J. Simpson, Christopher M. Thomas Chemistry & Biology Volume 10, Issue 5, Pages 419-430 (May 2003) DOI: 10.1016/S1074-5521(03)00091-7

Figure 1 Structure of Pseudomonic Acid A Pseudomonic acid consists of a C17 unit (monic acid) derived from an unsaturated polyketide containing a pyran ring, and a C9 saturated fatty acid (9-hydroxynonanoic acid). Chemistry & Biology 2003 10, 419-430DOI: (10.1016/S1074-5521(03)00091-7)

Figure 2 Organization of Mupirocin Biosynthesis Cluster Chemistry & Biology 2003 10, 419-430DOI: (10.1016/S1074-5521(03)00091-7)

Figure 3 Models for Biosynthesis of Mupirocin Components (A) Mupirocin biosynthesis model. (B) HMG-based model for incorporation of the C15 methyl group in monic acid. (C) 9-hydroxynonanoic biosynthesis model. (Ci) HMG starter unit formed from condensation of acetate and acetoacetate catalyzed by MupH and extended by two malonate condensations followed by removal of 3-methyl and hydroxyl groups. (Cii) Alternatively, a 3-hydroxyproprionate starter unit may be extended by three malonate condensations. ACP = acyl carrier protein; AT = acyl transferase; DH = dehydratase; HMG = 3-hydroxy-3-methylglutaric acid; KS = ketosynthase; MupE = enoyl reductase; MupH = HMG-CoA synthase; TE = thioesterase. Chemistry & Biology 2003 10, 419-430DOI: (10.1016/S1074-5521(03)00091-7)

Figure 4 HPLC Analysis of Mupirocin Production (A) P. fluorescens (NCIMB 10586). (B) NCIMBΔKR1. (C) Mupirocin standard (100 ng). Chemistry & Biology 2003 10, 419-430DOI: (10.1016/S1074-5521(03)00091-7)