Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,

Slides:

Advertisements

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Advertisements

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

Graft smooth muscle cells specifically synthesize increased collagen

Aortic injury occurring after minor trauma in ankylosing spondylitis

Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion

Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.

Impact of a critical pathway on postoperative length of stay and outcomes after infrainguinal bypass Andrew C. Stanley, MD, Maryann Barry, RN, Thayer.

Larry W. Kraiss, MDa,b,c, Neal M. Alto, BSa,c, Dan A

Somatostatin gene transfer and expression in endothelial cells

A new valvulotome for in situ bypass grafts

Aortic injury occurring after minor trauma in ankylosing spondylitis

Volume 114, Issue 3, Pages (March 1998)

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD

In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta William D. McMillan,

Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone C. Pross, MD,

Dennis R. Holmes, MD, William Wester, BA, Robert W

Richard R. Keen, MD, Kevin D

Elastase-induced matrix degradation in arterial organ cultures: An in vitro model of aneurysmal disease Andrew Wills, BSc, Matthew M. Thompson, MD, FRCS,

Mark F. Fillinger, MD, Susan E. O'Connor, MD, Robert J

Michael A. Golden, MD, Y. P. Tina Au, PhD, Richard D

Malcolm O. Perry, MD, Richard Kempczinski, MD

Recurrent thoracic outlet syndrome

The proliferative response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model David J.

Volume 54, Issue 4, Pages (October 1998)

Platelet-derived growth factor is a cofactor in the induction of 1α(I) procollagen expression by transforming growth factor β1 in smooth muscle cells

Aortic collagenase activity as affected by laparotomy, cecal resection, aortic mobilization, and aortotomy in rats Jon R. Cohen, M.D., Malcolm O. Perry,

Nitric oxide prevents p21 degradation with the ubiquitin-proteasome pathway in vascular smooth muscle cells Melina R. Kibbe, MD, Suhua Nie, MD, Dai-Wu.

Gabriele Di Luozzo, MD, Jaya Bhargava, PhD, Richard J. Powell, MD

Volume 62, Issue 1, Pages (July 2002)

Identification of multiple genes with altered expression at the distal anastomosis of healing polytetrafluoroethylene grafts Juan A. Cordero, MD, William.

Nitric oxide inhibition increases matrix metalloproteinase–9 expression by rat aortic smooth muscle cells in vitro Gilbert R. Upchurch, MD, John W. Ford,

Retroviral vector–mediated transfer and expression of human tissue plasminogen activator gene in human endothelial and vascular smooth muscle cells Daryoush.

James Majeski, MD, PhD, E. Stanley Crawford, MD, Elizabeth I

Ambient oxygen tension modulates endothelial fibrinolysis

Objective tinnitus resulting from internal carotid artery stenosis

Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth.

The Journal of Vascular Surgery: 1982 to 1990

The effect of growth factors, cytokines, and extracellular matrix proteins on fibronectin production in human vascular smooth muscle cells Terry L. Kaiura,

Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I

Renal transplant perfusion during aortoiliac aneurysmectomy

Treatment of venous disease – The innovators

Recreational weight lifting and aortic dissection: Case report

Calvin B. Ernst, MD Journal of Vascular Surgery

Ruptured abdominal aortic aneurysm: A population-based study

Mechanism of inhibition of matrix metalloproteinase-2 expression by doxycycline in human aortic smooth muscle cells Jian Liu, MD, Wanfen Xiong, PhD,

Cardiovascular surgery—The rocket and its stars: Presidential address

Sequential effects of high glucose on mesangial cell transforming growth factor-β1 and fibronectin synthesis Jong Hoon Oh, Hunjoo Ha, Mi Ra Yu, Hi Bahl.

Effect of hormonal agents on monocyte chemotactic protein-1 expression by endometrial epithelial cells of women with endometriosis Annie Boucher, M.S.,

Composite sequential bypasses to the ankle and beyond for limb salvage

Research and research funding in vascular surgery

Activation of integrin receptors is required for growth factor–induced smooth muscle cell dysfunction Kyotaro Mawatari, MD, Bo Liu, PhD, K.Craig Kent,

Alex Limanni, M. D. , Timothy Fleming, Ph. D. , Rodolfo Molina, M. D

Endotoxemia after major vascular operations

Septic embolism complicating infective endocarditis

Seizures following subclavian-carotid bypass

Acute aortic occlusion – Factors that influence outcome

The effects of endothelial injury on smooth muscle cell proliferation

TH2 Cytokine-enhanced and TGF-β-enhanced vascular endothelial growth factor production by cultured human airway smooth muscle cells is attenuated by IFN-γ.

Monitoring vascular surgical performance

D. Emerick Szilagyi, MD—An appreciation

Presidential address: The second-generation vascular surgeon

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Effects of somatostatin, somatostatin analogs, and endothelial cell somatostatin gene transfer on smooth muscle cell proliferation in vitro Rajabrata.

Fibroblasts cultured from venous ulcers display cellular characteristics of senescence Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD,

Popliteal entrapment syndrome: A report of tibial nerve entrapment

Cornelius A. Sullivan, MD, Michael J. Rohrer, MD, Bruce S. Cutler, MD

Presentation transcript:

Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani, MPH, DVM, Rajabrata Sarkar, MD, PhD, Theodore H. Welling, BS, Andrew Kriegel, MD, John W. Ford, BS, James C. Stanley, MD, Sem H. Phan, PhD, MD Journal of Vascular Surgery Volume 25, Issue 3, Pages 446-452 (March 1997) DOI: 10.1016/S0741-5214(97)70254-4 Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Effect of TGF-β1 on lysyl oxidase enzyme activity secreted into media by cultured rat aortic smooth muscle cells. Confluent cultures were arrested in serum-free media for 24 hours and subsequently incubated for additional 24 hours in fresh, serum-free medium supplemented with 0.1, 0.5, 1.0, and 10 ng/ml of TGF-β1 or control vehicle. Data are pooled mean ± SEM of β-aminoproprionitrile-inhibited activity from triplicate aliquots of conditioned media derived from triplicate cultures for each condition. BAPN, β-aminoproprionitrile; TGF, transforming growth factor. Journal of Vascular Surgery 1997 25, 446-452DOI: (10.1016/S0741-5214(97)70254-4) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Concentration-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells incubated for 24 hours in presence of 0.1, 0.5, 1.0, and 10 ng/ml of TGF-β1 or control vehicle. Each lane represents RNA sample from single culture. B, Radioactive image analysis of Northern blot in A. Data are pooled mean ± SEM of activities from three lanes per condition. Journal of Vascular Surgery 1997 25, 446-452DOI: (10.1016/S0741-5214(97)70254-4) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Concentration-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells incubated for 24 hours in presence of 0.1, 0.5, 1.0, and 10 ng/ml of TGF-β1 or control vehicle. Each lane represents RNA sample from single culture. B, Radioactive image analysis of Northern blot in A. Data are pooled mean ± SEM of activities from three lanes per condition. Journal of Vascular Surgery 1997 25, 446-452DOI: (10.1016/S0741-5214(97)70254-4) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Time-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth-muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P-labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells cultured in presence of 1 ng/ml. B, Radioactive image analysis of Northern blot in A. Data are mean ± SEM for 2 or 3 lanes per condition. Journal of Vascular Surgery 1997 25, 446-452DOI: (10.1016/S0741-5214(97)70254-4) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Time-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth-muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P-labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells cultured in presence of 1 ng/ml. B, Radioactive image analysis of Northern blot in A. Data are mean ± SEM for 2 or 3 lanes per condition. Journal of Vascular Surgery 1997 25, 446-452DOI: (10.1016/S0741-5214(97)70254-4) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions