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Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I

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Presentation on theme: "Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I"— Presentation transcript:

1 Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells 
Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I. Willis, MD, George P. Tuszynski, PhD, Bauer E. Sumpio, MD, PhD  Journal of Vascular Surgery  Volume 29, Issue 6, Pages (June 1999) DOI: /S (99) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig 1 Growth-arrested confluent vascular smooth muscle cells were treated with thrombospondin-1 (20 μg/mL) for 0, 15, 30, and 120 minutes. Cell lysates were subjected to antiphosphotyrosine (RC20H) immunoblotting. Three prominent bands were identified at 68 kDa, 125 kDa, and 180 kDa. Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig 2 Growth-arrested vascular smooth muscle cells were treated with thrombospondin-1 (20 μg/mL) for 15, 30, and 120 minutes. Serum-free medium was the negative control. Cell lysates were subjected to immunoprecipitation with anti-focal adhesion kinase antibodies. A, Antiphosphotyrosine immunoblotting demonstrates tyrosine phosphorylation of focal adhesion kinase, hence activation. B, Immunoblotting with anti-focal adhesion kinase antibodies confirms equal loading of protein and no change in focal adhesion kinase levels. Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig 3 Phosphorylation of focal adhesion kinase quantified by means of densitometry in four different experiments. Normalized data demonstrates enhanced tyrosine phosphorylation of focal adhesion kinase for thrombospondin-1 stimulated vascular smooth muscle cells, compared with cells in serum-free medium (P < .05), and was no different from cells exposed to 10 ng/mL platelet-derived growth factor (positive control). Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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