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Fibroblasts cultured from venous ulcers display cellular characteristics of senescence  Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD,

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Presentation on theme: "Fibroblasts cultured from venous ulcers display cellular characteristics of senescence  Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD,"— Presentation transcript:

1 Fibroblasts cultured from venous ulcers display cellular characteristics of senescence 
Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD, Karen Shon, Tania Phillips, MD, James O. Menzoian, MD  Journal of Vascular Surgery  Volume 28, Issue 5, Pages (November 1998) DOI: /S (98) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Correlation between percentage of SA-β-Gal positive cells and the ratio between normal fibroblasts (normal-fb) and fibroblasts cultured from venous ulcers (wound-fb) growth rates. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Cellular fibronectin protein extracted from 80% to 90% confluent third passage fibroblasts isolated from venous ulcers (wound-fb) and uninvolved skin (normal-fb) of the ipsilateral thigh of the same patients. Densitometry was performed on scans from immunoblot autoradiograph using image analysis software. Results are presented in the table. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Cellular fibronectin messenger RNA (mRNA) extracted from 80% to 90% confluent third passage fibroblasts isolated from venous ulcers (wound-fb) and uninvolved skin (normal-fb) of the ipsilateral thigh of the same patients. Ethidium bromide labeled 18S subunit of total RNA loaded into each lane was used to calculate the ratio between cellular fibronectin mRNA and total RNA for purposes of comparison. Densitometry was performed on scans from Northern blot autoradiograph and picture of ethidium bromide labeled gel using image analysis software. Results are presented in table. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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