Allergy caused by ingestion of zucchini (Cucurbita pepo) : Characterization of allergens and cross-reactivity to pollen and other foods Jürgen Reindl,

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Presentation transcript:

Allergy caused by ingestion of zucchini (Cucurbita pepo) : Characterization of allergens and cross-reactivity to pollen and other foods Jürgen Reindl, MSca, Mark D. Anliker, MDb, Fariba Karamloo, PhDa, Stefan Vieths, PhDa, Brunello Wüthrich, MDb Journal of Allergy and Clinical Immunology Volume 106, Issue 2, Pages 379-385 (August 2000) DOI: 10.1067/mai.2000.107602 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 A, Dose-related EAST inhibitions with sera from patients 148, 225, and 291: inhibition with plant extracts. Inhibitors are as follows: filled circles, zucchini; open circles, watermelon; filled squares, birch; open squares, ragweed; filled triangles, grass; open triangles, mugwort; filled diamonds, latex; and ×, skim milk. B, Dose-related EAST inhibitions with sera from patients 148, 225, and 291: inhibition with recombinant allergens and glycopeptides. Inhibitors are as follows: filled circles, rBet v 2; open circles, rBet v 1; filled triangles, bromelain glycopeptide; and open triangles, fibrin glycopeptide. Journal of Allergy and Clinical Immunology 2000 106, 379-385DOI: (10.1067/mai.2000.107602) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 Blot-inhibition experiments with sera from patients 148, 225, 291, and 652. Inhibitors are as follows: lane 1, no inhibitor; lane 2, 100 μg of protein from zucchini extract; lane 3, 10 μg of bromelain glycopeptide; lane 4, 10 μg of fibrin glycopeptide; lane 5, 100 μg of protein from birch extract; lane 6, 10 μg of rBet v 2; lane 7, 100 μg of protein from melon extract; and lane 8, 100 μg of protein from skim milk extract. Journal of Allergy and Clinical Immunology 2000 106, 379-385DOI: (10.1067/mai.2000.107602) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 Optimized blot inhibition with serum 291. Inhibitors are as follows: lane 1, no inhibitor; lane 2, 100 μg of protein from zucchini extract; lane 3, 100 μg of protein from birch extract; lane 4, 10 μg of rBet v 2; and lane 5, 100 μg of protein from skim milk extract. Journal of Allergy and Clinical Immunology 2000 106, 379-385DOI: (10.1067/mai.2000.107602) Copyright © 2000 Mosby, Inc. Terms and Conditions