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Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin) 

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Presentation on theme: "Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin) "— Presentation transcript:

1 Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)  Christof Ebner, MDa, Reinhold Hirschwehr, MDa, Leonhardt Bauer, MDa, Heimo Breiteneder, PhDa, Rudolf Valenta, MDa, Herwig Ebner, MDb, Dietrich Kraft, MDa, Otto Scheiner, PhDa  Journal of Allergy and Clinical Immunology  Volume 95, Issue 5, Pages (May 1995) DOI: /S (95)70096-X Copyright © 1995 Mosby, Inc. Terms and Conditions

2 FIG. 1 A, IgE immunoblots with sera from 20 patients with birch pollen allergy on birch pollen-coated nitrocellulose strips. B, IgE immunoblots with sera from 20 patients with birch pollen allergy on apple extract-coated nitrocellulose strips. C, IgE immunoblots with sera from 20 patients with birch pollen allergy on pear extract-coated nitrocellulose strips. D, IgE immunoblots with sera from 20 patients with birch pollen allergy on celery extract-coated nitrocellulose strips. E, IgE immunoblots with sera from 20 patients with birch pollen allergy on carrot extract-coated nitrocellulose strips. F, IgE immunoblots with sera from 20 patients with birch pollen allergy on potato extract-coated nitrocellulose strips. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

3 FIG. 1 A, IgE immunoblots with sera from 20 patients with birch pollen allergy on birch pollen-coated nitrocellulose strips. B, IgE immunoblots with sera from 20 patients with birch pollen allergy on apple extract-coated nitrocellulose strips. C, IgE immunoblots with sera from 20 patients with birch pollen allergy on pear extract-coated nitrocellulose strips. D, IgE immunoblots with sera from 20 patients with birch pollen allergy on celery extract-coated nitrocellulose strips. E, IgE immunoblots with sera from 20 patients with birch pollen allergy on carrot extract-coated nitrocellulose strips. F, IgE immunoblots with sera from 20 patients with birch pollen allergy on potato extract-coated nitrocellulose strips. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

4 FIG. 1 A, IgE immunoblots with sera from 20 patients with birch pollen allergy on birch pollen-coated nitrocellulose strips. B, IgE immunoblots with sera from 20 patients with birch pollen allergy on apple extract-coated nitrocellulose strips. C, IgE immunoblots with sera from 20 patients with birch pollen allergy on pear extract-coated nitrocellulose strips. D, IgE immunoblots with sera from 20 patients with birch pollen allergy on celery extract-coated nitrocellulose strips. E, IgE immunoblots with sera from 20 patients with birch pollen allergy on carrot extract-coated nitrocellulose strips. F, IgE immunoblots with sera from 20 patients with birch pollen allergy on potato extract-coated nitrocellulose strips. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

5 FIG. 1 A, IgE immunoblots with sera from 20 patients with birch pollen allergy on birch pollen-coated nitrocellulose strips. B, IgE immunoblots with sera from 20 patients with birch pollen allergy on apple extract-coated nitrocellulose strips. C, IgE immunoblots with sera from 20 patients with birch pollen allergy on pear extract-coated nitrocellulose strips. D, IgE immunoblots with sera from 20 patients with birch pollen allergy on celery extract-coated nitrocellulose strips. E, IgE immunoblots with sera from 20 patients with birch pollen allergy on carrot extract-coated nitrocellulose strips. F, IgE immunoblots with sera from 20 patients with birch pollen allergy on potato extract-coated nitrocellulose strips. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

6 FIG. 1 A, IgE immunoblots with sera from 20 patients with birch pollen allergy on birch pollen-coated nitrocellulose strips. B, IgE immunoblots with sera from 20 patients with birch pollen allergy on apple extract-coated nitrocellulose strips. C, IgE immunoblots with sera from 20 patients with birch pollen allergy on pear extract-coated nitrocellulose strips. D, IgE immunoblots with sera from 20 patients with birch pollen allergy on celery extract-coated nitrocellulose strips. E, IgE immunoblots with sera from 20 patients with birch pollen allergy on carrot extract-coated nitrocellulose strips. F, IgE immunoblots with sera from 20 patients with birch pollen allergy on potato extract-coated nitrocellulose strips. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

7 FIG. 1 A, IgE immunoblots with sera from 20 patients with birch pollen allergy on birch pollen-coated nitrocellulose strips. B, IgE immunoblots with sera from 20 patients with birch pollen allergy on apple extract-coated nitrocellulose strips. C, IgE immunoblots with sera from 20 patients with birch pollen allergy on pear extract-coated nitrocellulose strips. D, IgE immunoblots with sera from 20 patients with birch pollen allergy on celery extract-coated nitrocellulose strips. E, IgE immunoblots with sera from 20 patients with birch pollen allergy on carrot extract-coated nitrocellulose strips. F, IgE immunoblots with sera from 20 patients with birch pollen allergy on potato extract-coated nitrocellulose strips. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

8 FIG. 2 Inhibition experiment. Lane 1, Sera from patients reactive with Bet v 1, Bet v 2, and the putative cross-reacting allergens in fruit and vegetable extracts were pooled and tested for IgE binding to birch, apple, pear, celery, carrot, and potato extracts. Lane 2, Immunoblot with the Bet v 1/Bet v 2-positive serum pool after preincubation with 10 μg of recombinant Bet v 1. Lane 3, Immunoblot with the Bet v 1/Bet v 2-positive serum pool after preincubation with 10 μg of recombinant Bet v 2. Lanes N and C, Normal human serum and buffer controls. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

9 FIG. 3 Immunoblot with an anti-Bet v 1 mAb. Reactivity with fruit and vegetable extracts. C, Control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

10 FIG. 4 Immunoblot with an anti-celery-profilin antibody from rabbit. Reactivity with fruit and vegetable extracts. C, Control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95)70096-X) Copyright © 1995 Mosby, Inc. Terms and Conditions

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