Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling.

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Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling two separate digests with tag 114 and 115, respectively, and combining the reaction mixtures in a 1:1 ratio.A, isotopic distribution of a doubly charged precursor [M + 2H]2+, m/z 598.8164. Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling two separate digests with tag 114 and 115, respectively, and combining the reaction mixtures in a 1:1 ratio.A, isotopic distribution of a doubly charged precursor [M + 2H]2+, m/z 598.8164. B, low mass region showing the signature ions used for quantification. The peptide was labeled by isobaric tags at the N terminus. William J. Snelling et al. Mol Cell Proteomics 2007;6:346-355 © 2007 The American Society for Biochemistry and Molecular Biology