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Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;4.A, MS/MS spectrum of singly phosphorylated 277ALGSFGSFGSFRSFA291 (m/z 1617.55).

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Presentation on theme: "Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;4.A, MS/MS spectrum of singly phosphorylated 277ALGSFGSFGSFRSFA291 (m/z 1617.55)."— Presentation transcript:

1 Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;4.A, MS/MS spectrum of singly phosphorylated 277ALGSFGSFGSFRSFA291 (m/z ). b5 and y11 ions allowed identification of Ser280 as the phosphorylated residue. Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;4.A, MS/MS spectrum of singly phosphorylated 277ALGSFGSFGSFRSFA291 (m/z ). b5 and y11 ions allowed identification of Ser280 as the phosphorylated residue. B, MS/MS spectrum of the corresponding diphosphorylated peptide (m/z ). y4, y5, y7, and y9 ions allowed identification of phosphorylated Ser280 and Ser283. C, MS/MS spectrum of the corresponding triphosphorylated peptide (m/z ) that is a mixture of two forms: b7 ion allowed determination of the phosphorylation of the two residues Ser280 and Ser283; y4, y5, and y6 ions indicated that Ser286 can be phosphorylated; y4bis, y5bis, and y6 showed that alternatively Ser289 can carry the third phosphorylation. *, pS, [MH]+, [MH − P]+, [MH − P − 18]+, [MH − 2P − 18]+, and [MH − 2P − 2 × 18]+ are as explained in the legend of Fig. 2. Sodana Prak et al. Mol Cell Proteomics 2008;7: © 2008 The American Society for Biochemistry and Molecular Biology

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