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Relative quantification of cis and trans PSP gp10040–42/47–52 variants

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Presentation on theme: "Relative quantification of cis and trans PSP gp10040–42/47–52 variants"— Presentation transcript:

1 Relative quantification of cis and trans PSP gp10040–42/47–52 variants.
Relative quantification of cis and trans PSP gp10040–42/47–52 variants.A, To estimate the relative in vitro efficiency of cis and trans PCPS, the peptide gp10040–52 and its heavy analog with amino acids 13C6-Lys, 13C6–15N-Leu, and 13C5–15N-Glu (RTK+6AWNRQL+7YPE+6W) were digested for 24 h by different 20S proteasomes and the gp10040–42/47–52 variants generated by cis (-A, -D) and trans (-B, -C) PCPS were investigated. B, All four peptides were detected by LC-MALDI-TOF-MS. As representative the mass spectrum of the gp10040–42/47–52 variants produced by T S proteasomes are reported (for MS/MS spectra see supplemental Fig. S11). Upper right, the MS intensity is noted. Michele Mishto et al. Mol Cell Proteomics 2012;11: © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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