Loss of Frzb and Sfrp1 differentially affects joint homeostasis in instability-induced osteoarthritis S. Thysen, F.P. Luyten, R.J. Lories Osteoarthritis.

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Loss of Frzb and Sfrp1 differentially affects joint homeostasis in instability-induced osteoarthritis S. Thysen, F.P. Luyten, R.J. Lories Osteoarthritis and Cartilage Volume 23, Issue 2, Pages 275-279 (February 2015) DOI: 10.1016/j.joca.2014.10.010 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Loss of Frzb increases cartilage damage in DMM model of OA. (A) Representative frontal hematoxylin-Safranin O stained sections of wild-type, Frzb−/− and Sfrp1−/− knees (medial condyle) in the DMM model or sham-operated. Bar = 100 μM, original magnification 20×. Arrows indicate cartilage fibrillation, triangles indicate loss of proteoglycans and asterisks indicate loss of cartilage. (B) Quantification of articular cartilage erosion in wild-type, Frzb−/− and Sfrp1−/− mice, subjected to either sham or DMM. The medial condyle of tibia and femur were evaluated by the OARSI scoring system. Data are shown as the maximum histologic score +95% CI. Left: WT sham-operated (n = 13), WT DMM (n = 12), Frzb−/− sham-operated (n = 13) and Frzb−/− DMM (n = 16). 2-way ANOVA P < 0.05 for genotype, intervention and interaction – P < 0.05 between Frzb−/− and littermates (Sidak test). Right: WT sham-operated (n = 18), WT DMM (n = 16), Sfrp1−/− sham-operated (n = 6) and Sfrp1−/− DMM (n = 10). Osteoarthritis and Cartilage 2015 23, 275-279DOI: (10.1016/j.joca.2014.10.010) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Loss of Sfrp1 increases subchondral bone plate thickness in DMM model of OA. (A) Representative frontal hematoxylin-Safranin O stained sections of wild-type, Frzb−/− and Sfrp1−/− knees (medial condyle) in the DMM model or sham-operated. Blue line delineates the subchondral bone area and white lines delineate the subchondral bone plate thickness. Bar = 100 μM, original magnification 10×. (B) Quantification of the subchondral bone plate. Data are shown as the mean ratio of subchondral bone plate area/subchondral bone area + 95% CI. Left: WT sham-operated (n = 20), WT DMM (n = 16), Frzb−/− sham-operated (n = 12) and Frzb−/− DMM (n = 13) (2-way ANOVA P < 0.05 for intervention only). Right: WT sham-operated (n = 20), WT DMM (n = 16), Sfrp1−/− sham-operated (n = 5) and Sfrp1−/− DMM (n = 11) (2-way ANOVA P < 0.05 for intervention and interaction). Osteoarthritis and Cartilage 2015 23, 275-279DOI: (10.1016/j.joca.2014.10.010) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Supplementary Fig. 1 Frzb and beta-catenin are present in the articular cartilage. Immunohistochemistry for Frzb (A) and total beta-catenin (B) on frontal sections of wild-type, Frzb−/− and Sfrp1−/− knees (medial condyle) in the DMM model or sham-operated. Bar = 100 μM, original magnification 20×. Osteoarthritis and Cartilage 2015 23, 275-279DOI: (10.1016/j.joca.2014.10.010) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Supplementary Fig. 2 Frzb and beta-catenin in the articular cartilage and the subchondral bone. Immunohistochemistry for Frzb (A) and total beta-catenin (B) on frontal sections of wild-type, Frzb−/− and Sfrp1−/− knees (medial condyle) in the DMM model or sham-operated. M, Meniscus. AC, articular cartilage. SB, subchondral bone. Bar = 200 μM, original magnification 10×. Osteoarthritis and Cartilage 2015 23, 275-279DOI: (10.1016/j.joca.2014.10.010) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions