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Upregulation of Atrogin-1/FBXO32 is not necessary for cartilage destruction in mouse models of osteoarthritis  H.-E. Kim, J. Rhee, S. Park, J. Yang, J.-S.

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Presentation on theme: "Upregulation of Atrogin-1/FBXO32 is not necessary for cartilage destruction in mouse models of osteoarthritis  H.-E. Kim, J. Rhee, S. Park, J. Yang, J.-S."— Presentation transcript:

1 Upregulation of Atrogin-1/FBXO32 is not necessary for cartilage destruction in mouse models of osteoarthritis  H.-E. Kim, J. Rhee, S. Park, J. Yang, J.-S. Chun  Osteoarthritis and Cartilage  Volume 25, Issue 3, Pages (March 2017) DOI: /j.joca Copyright © 2016 The Author(s) Terms and Conditions

2 Fig. 1 Atrogin-1 is upregulated in OA chondrocytes and cartilage. (A) Atrogin-1 mRNA levels were quantified by qRT-PCR in chondrocytes stimulated with IL-1β (n = 8) or infected with control adenovirus (Ad-C; MOI, 800) or the indicated MOI of Ad-Epas1 (n = 8), Ad-Zip8 (n = 8), and Ad-Mtf1 (n = 8). (B and C) Representative images of Alcian blue and immunohistochemical staining for Atrogin-1 in human OA cartilage (B; n = 5) and safranin-O staining and immunostaining for Atrogin-1 in cartilage sections from mice subjected to DMM surgery or IA injection of Ad-C (n = 5), Ad-Epas1 (n = 5), or Ad-Zip8 (C) (n = 5). Values are the means ±95% CI. Scale bar: 50 μm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 The Author(s) Terms and Conditions

3 Fig. 2 Upregulation of Atrogin-1 is not sufficient or necessary for OA cartilage destruction in mice. (A) The mRNA and protein levels of the indicated molecules were detected in chondrocytes infected with the indicated MOI of Ad-Fbxo32. (B) Representative images of Atrogin-1 immunostaining (left) and safranin-O staining (right) in cartilage sections from mice subjected to IA injection with Ad-C (n = 8) or Ad-Fbxo32 (n = 8). (C) Representative images of H&E staining in knee joint sections from mice subjected to IA injection with Ad-C (n = 8) or Ad-Fbxo32 (n = 8). (D) Representative images of Atrogin-1 immunostaining in cartilage sections of wild-type (WT; n = 5) and Fbxo32-knockout (KO) mice (n = 5). (E) Genotype and mRNA levels of Atrogin-1 in chondrocytes of WT and Fbxo32 KO mice. (F) Representative images of safranin-O staining, OARSI grade, and osteophyte maturity in sham- and DMM-operated wild-type (WT; n = 8) and Fbxo32−/− (n = 8). Values are the means ±95% CI. Scale bar: 50 μm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 The Author(s) Terms and Conditions

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