1. Intra-articular injection of interleukin-4 decreases nitric oxide production by chondrocytes and ameliorates subsequent destruction of cartilage in instability-induced osteoarthritis in rat knee joints 
M. Yorimitsu, M.D., K. Nishida, M.D., Ph.D., A. Shimizu, M.D., Ph.D., H. Doi, M.D., S. Miyazawa, M.D., Ph.D., T. Komiyama, M.D., Ph.D., Y. Nasu, M.D., A. Yoshida, S. Watanabe, M.D., Ph.D., T. Ozaki, M.D., Ph.D. 
Osteoarthritis and Cartilage 
Volume 16, Issue 7, Pages (July 2008)
DOI: /j.joca
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
Real-time PCR of iNOS mRNA expression. Chondrocytes were obtained from the knee joints of 7-day-old Wistar rats and cultured under the load of CTS (0.5Hz and 7% elongation), or no stress, for 24 and 48h, with or without treatment of rrIL-4 (rrIL-4; 1 or 10ng/ml). Data are shown as the mean±SD. Statistical significance: *P<0.001 vs no CTS, ‡P<0.005, **P<0.001 vs 24 or 48h of CTS without rrIL-4 treatment, †P<0.001 vs 24h of CTS with treatment of 1ng/ml of rrIL-4.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Representative histologic appearances of medial femoral condyle cartilage (safranin O staining) of the sham operation group (a–c), the control group (d–f) and the treatment group (rrIL-4; 50ng/joint/day; g–i) 2 (a, d, and g), 4 (b, e, and h), and 6 (c, f, and i) weeks after surgery.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
Representative results of immunohistochemistry for aggrecan neoepitope (NITEGE) in femoral condylar cartilage of knee joints of OA control (a–c) and IL-4 treated (d–f) animals at 2 weeks (a and d), 4 weeks (b and e), and 6 weeks (c and f) after surgery. Original magnification: 200×.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
(A) Representative appearances of immunohistochemistry for NT in medial femoral condylar cartilage of the sham operation group (a–c), the control group (d–f), and the treatment group (rrIL-4; 50ng/joint/day; g–i) 2 (a, d, and g), 4 (b, e, and h), and 6 (c, f, and i) weeks after surgery. (B) Population of NT-positive chondrocytes in medial femoral condylar cartilage of the sham operation group, the control group, and the treatment group (rrIL-4; 50ng/joint/day). Data are expressed as the mean±SD. Statistical significance: *P<0.005, **P<0.001 vs the sham operation group and †P<0.05 vs the control group.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions