Unified Solo vectors for mutagenesis in C. albicans.

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Target gene gRNA NameTarget sequence / PAM*Restriction Enzyme Young Seedling Albino (YSA)gYSAGCGCGCCACCTCGGCCGAAG / CGGSfiI Phytoene desaturase (PDS) gPDS-1†CGTCCAACCCATTCCTCTGC.

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Presentation transcript:

Unified Solo vectors for mutagenesis in C. albicans. Unified Solo vectors for mutagenesis in C. albicans. (A) Unified Solo vector integrated at Neut5L directed against ADE2 results in translocations detectable by PCR with the indicated primers (red and green arrows). (B) Gene-drive-based Unified Solo vector to target CRISPR to delete ADE2. Vector pV1534 is linearized in vitro with BsmBI, while vector pV1535 is linearized in vivo by CRISPR. (C) Transformation of SC5314 with circular pV1534 (left), BsmBI-linearized pV1534 (middle), or circular pV1535 (right). (D) Maltose-induced gene drive targeting ADE2. Heterozygous mutants can be induced to create homozygous mutants by growth in maltose medium. Valmik K. Vyas et al. mSphere 2018; doi:10.1128/mSphere.00154-18