1. Side-view and apical-view projections of mutant and validation strain biofilms.
Side-view and apical-view projections of mutant and validation strain biofilms. The WT SN250 strain, ume6Δ/Δ mutant, brg1Δ/Δ mutant, ume6Δ/Δ brg1Δ/Δ double mutant, and UME6:BRG1 validation strains were assayed for phenotypic rescue of biofilm formation. (A) Lateral projections of C. albicans WT, mutant, and validation strain biofilms. A wild-type strain forms a large robust biofilm, while the ume6Δ/Δ, brg1Δ/Δ, and ume6Δ/Δ brg1Δ/Δ mutant strains form defective biofilms. The UME6:BRG1 validation strain forms a biofilm with more than half the thickness of the WT biofilm. (B) Apical-view projections of representative sections of each biofilm. A wild-type strain forms a robustly filamentous biofilm, whereas ume6Δ/Δ, brg1Δ/Δ, and ume6Δ/Δ brg1Δ/Δ mutant strains form biofilms with largely reduced filamentation. The UME6:BRG1 strain forms a biofilm with visibly filamentous cells.
Manning Y. Huang et al. mSphere 2018; doi: /mSphere