Ubiquitous structures responsible for IgE cross-reactivity between tomato fruit and grass pollen allergens Arnd Petersen, PhDa, Stefan Vieths, PhDb,

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Presentation transcript:

Ubiquitous structures responsible for IgE cross-reactivity between tomato fruit and grass pollen allergens Arnd Petersen, PhDa, Stefan Vieths, PhDb, Holger Aulepp, MDc, Max Schlaak, MDa, Wolf-Meinhard Becker, PhDa Journal of Allergy and Clinical Immunology Volume 98, Issue 4, Pages 805-815 (October 1996) DOI: 10.1016/S0091-6749(96)70130-6 Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 1 Binding patterns of patients’ IgE to tomato fruit extract after Western blotting. MW, Molecular weight; M, molecular weight marker: I, protein staining by India ink; 0, buffer control; N, healthy control serum; Bo 64 - Bo 131, individual patient sera; P1, rabbit antiserum raised against celery profilin; P2, rabbit antiserum raised against ragweed profilin. Journal of Allergy and Clinical Immunology 1996 98, 805-815DOI: (10.1016/S0091-6749(96)70130-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 Identification of carbohydrate-directed patient’s IgE to tomato fruit extract. After Western blotting, nitrocellulose strips were treated as follows: U = untreated, C = treated with sorbitol-inactivated NaIO 4 solution, and P = treated with 50 mmol/L NaIO 4 solution. Binding patterns of individual patient sera (Bo 64 - Bo 135) and of one healthy control (NSB) are shown. MW, Molecular weight. Journal of Allergy and Clinical Immunology 1996 98, 805-815DOI: (10.1016/S0091-6749(96)70130-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Immunoblot inhibition test of tomato fruit (left) and timothy grass pollen extract (right). Inhibitors (ovalbumin, timothy grass pollen, and tomato fruit extract) were preincubated at concentrations of 250 μg/ml of serum Bo 64 (diluted 1:25). O, Ovalbumin; G, timothy grass pollen; T, tomato fruit extract; I, protein staining by India ink; P, serum Bo 64 without inhibitor; A, Aleuria aurantia agglutinin. Journal of Allergy and Clinical Immunology 1996 98, 805-815DOI: (10.1016/S0091-6749(96)70130-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Two-dimensional PAGE blotting for the identification and characterization of single components of tomato fruit extract. A, IgE reactivity of a patient’s serum. B, Detection by AAA. (Protein marked in A was investigated by N-terminal microsequencing.) Journal of Allergy and Clinical Immunology 1996 98, 805-815DOI: (10.1016/S0091-6749(96)70130-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Two-dimensional PAGE blotting for the identification and characterization of single components of tomato fruit extract. A, IgE reactivity of a patient’s serum. B, Detection by AAA. (Protein marked in A was investigated by N-terminal microsequencing.) Journal of Allergy and Clinical Immunology 1996 98, 805-815DOI: (10.1016/S0091-6749(96)70130-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 5 Determination of glycan binding sites by ELISA with glycopeptides. Bromelain from pineapple (Brl), chemically defucosylated bromelain (Brl-F), and bovine fibrin (Fib) served as antigens. Serum of patient Bo 64 was diluted stepwise (1:3). Journal of Allergy and Clinical Immunology 1996 98, 805-815DOI: (10.1016/S0091-6749(96)70130-6) Copyright © 1996 Mosby, Inc. Terms and Conditions