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Identification of IgE Journal of Allergy and Clinical Immunology

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Presentation on theme: "Identification of IgE Journal of Allergy and Clinical Immunology"— Presentation transcript:

1 Identification of IgE Journal of Allergy and Clinical Immunology
Kimishige Ishizaka, MD, PhD, Teruko Ishizaka, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 137, Issue 6, Pages (June 2016) DOI: /j.jaci Copyright © 2016 The Authors Terms and Conditions

2 Fig 1 Detection of anti-reagin antibodies by PK reactions. For passive sensitization, 0.05 mL of 1:100 (bottom) to 1:800 (top) dilutions of a mixture of the patient's serum with either antiserum (Abs) or normal rabbit serum (Cont) were injected (see text). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

3 Fig 2 Distribution of γE antibodies against ragweed antigen in chromatographic fractions of the sera of ragweed-sensitive patients from the DEAE cellulose column. Stepwise elution was made with 0.005 mol/L (I), 0.025 mol/L (II), 0.035 mol/L (III), 0.05 mol/L (IV), and 0.08 mol/L (V) phosphate buffers, pH 8.0. S, Original serum. The center well of the agarose plate was filled with anti-reagin (γE) antiserum, followed by 131I-labeled ragweed antigen E. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

4 Fig 3 Radioimmunoelectrophoresis of the reagin-rich fraction of the serum of a ragweed-sensitive patient with hay fever. After electrophoresis, the trough was filled with anti-γE, followed by 131I-labeled ragweed antigen E. Both the stained slide (top) and autoradiograph (bottom) are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

5 Fig 4 Radioimmunodiffusion analysis of γE isolated from the serum of a ragweed-sensitive patient. The center well was filled with purified γE. Peripheral wells were filled with the antiserum specific for each immunoglobulin isotype, followed by 131I-labeled ragweed antigen E. Both the stained slide (left) and autoradiograph (right) are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

6 Fig 5 Antigenic identity of γE and myeloma protein ND. Purified γE from the sera of ragweed-sensitive patients was placed in the center well. Peripheral wells were filled with the antiserum specific for ND protein and the antibodies specific for each human immunoglobulin isotype, followed by 131I-labeled ragweed antigen E. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

7 Fig 6 Identification of IgE on human basophilic granulocytes: autoradiograph of normal human leukocytes treated with 125I-labeled anti-IgE. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

8 Fig 7 Selective binding of IgE on monkey mast cells. E myeloma protein PS was injected intracutaneously into a monkey. Twenty-four hours later, the skin tissues were treated with 125I-labeled anti-IgE, and frozen sections of the tissues were examined. Both the stained slide (bottom) and autoradiograph (top) are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

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