Both AtSYP121-Sp2 and ZmSYP121-Sp2 Fragments Selectively Reduce the Accumulation of Zm-PIP2;5 in the Plasma Membrane of Maize Mesophyll Protoplasts.(A)

Slides:

Advertisements

Similar presentations

Reliable and Global Measurement of Fluorescence Resonance Energy Transfer Using Fluorescence Microscopes Zongping Xia, Yuechueng Liu Biophysical Journal

Advertisements

Quantification of intracellular fuzzy staining (A) and of spherical bodies (B) in root cells upon an NaCl treatment. Quantification of intracellular fuzzy.

GFP Chlorophyll Merged A B C D

Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22.

Synaptotagmin SYTA Forms ER-Plasma Membrane Junctions that Are Recruited to Plasmodesmata for Plant Virus Movement Amit Levy, Judy Y. Zheng, Sondra G.

Volume 9, Issue 5, Pages (May 2016)

Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling by Yong Zhou, Ching-On Wong, Kwang-jin Cho, Dharini van der Hoeven,

MAPKKK5-induced cell death is independent of Ser-289 phosphorylation.

Regulatory CD4+ T cell–derived IL-10 is important for B cell differentiation and the GC response. Regulatory CD4+ T cell–derived IL-10 is important for.

STARD3 favors cholesterol accumulation in endosomes

The ER is the main source of cholesterol accumulated by STARD3 in endosomes The ER is the main source of cholesterol accumulated by STARD3 in endosomes.

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

HURP is an obstacle for KIF18A on K-fibers in metaphase cells.

Volume 19, Issue 12, Pages (June 2017)

Expression and Histone H4 Acetylation Levels of MAPK6 and WRKY53 in the HDT701 OX and RNAi Plants after M. oryzae Inoculation. Expression and Histone H4.

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

The Subcellular Distribution of a Sar1-YFP Fusion Is Altered by the Expression of Golgi Markers.(A) and (B) Cytoplasm of a tobacco leaf epidermal cell.

Histological and cellular localization of CsSUT1.

Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants.

Cyclic electron flow in hpr1 mutants.

Mitochondrial enzymatic activities and root tip respiration in wild type (WT) and mab1-1. Mitochondrial enzymatic activities and root tip respiration in.

Thermomorphogenesis in seedling organs is autonomous or interdependent

Numerical analysis of cell morphology of tapetal cells in wild type.

The SI-Induced Increase in PA Levels Is Mediated by PbrPLDδ1 in Pollen

Self-Incompatible Pollen Tubes Exhibit Increased PA Accumulation.

Aggregation is not required for cytoplasmic relocalization induced by misfolding mutations. Aggregation is not required for cytoplasmic relocalization.

Absence of miR‐21 in macrophages promotes foam cell formation

Fig. 2. Proportion of motile objects and track length

Chlorophyll concentration (conc

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

dex-1 expression in the seam cells is regulated by DAF-16.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

Fig. 3. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC.

Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

Drs expression profiles for OreR flies infected with the various C

Clade I TGAs are required for BOP1 induction of ATH1 expression.

FRAP and FLIP analysis of GFP-ADAR2 and GFP-ADAR1C-Term.

The BRCA1 aggregates exclude large nuclear structures.

Single Mutations in the Transmembrane Domains of Maize Plasma Membrane Aquaporins Affect the Activity of Monomers within a Heterotetramer Marie C. Berny,

Mice fed GP-SPI diet show improved fasting glucose and oral glucose tolerance. Mice fed GP-SPI diet show improved fasting glucose and oral glucose tolerance.

SAM transport by Gap4 in S. cerevisiae cells.

Volume 28, Issue 3, Pages e4 (February 2018)

SlMYB21 Encodes an Active Transcription Factor That Interacts With SlJAZ9 and Complements the Arabidopsis Mutant myb21-5. SlMYB21 Encodes an Active Transcription.

ERF12 Binds Directly to the DRE/CRT Element in the DOG1 Promoter.

TDIF-Sensitive Xylem Formation in wox4 Mutants

Influence of RdDM on DCL4 Transcript Isoform Expression.

The presence of soluble IL12p70 during moDCpoly stimulation of KIRnegNKG2Aneg cells results in a dominant population of multifunctional NKG2Apos NK cells.

Light-Dependent Lesions in Ufo1-1 Leaves Have Elevated Sucrose and Starch Levels. Light-Dependent Lesions in Ufo1-1 Leaves Have Elevated Sucrose and Starch.

Relative Protein Quantification of CcmK4, RbcS, and CcmM in the Carboxysome under Different CO2 Levels and Light Intensities using Confocal Microscopy.

Osmotic Stress Affects S6K1 Kinase Activity. (A) Leaves of tobacco (N

OsbZIP46 Binds to the OsPM1 Promoter and Activates OsPM1 Expression in Vivo. OsbZIP46 Binds to the OsPM1 Promoter and Activates OsPM1 Expression in Vivo.

Volume 7, Issue 7, Pages (July 2014)

Akt overexpression rescues Aβ42-induced toxicity without affecting levels of intraneuronal Aβ. Akt overexpression rescues Aβ42-induced toxicity without.

Quantification of TUNEL staining and OS length among the rab11a constructs. Quantification of TUNEL staining and OS length among the rab11a constructs.

Cell Sorting of Induced SE-like Cells Using SEOR1pro:SEOR1-YFP.

Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

The channel-kinase TRPM7 regulates antigen gathering and internalization in B cells by Mithunah Krishnamoorthy, Laabiah Wasim, Fathima Hifza Mohamed Buhari,

Analysis of diTPSs from Stevia.

In Vivo Interaction Between AtPIP2;1 and GRFs

ROS Generation, Defense Gene Expression, and Histone Acetylation Levels in the HDT701 OX and RNAi Plants.(A) ROS level in HDT701 RNAi plants after flg22.

Induction of Autophagy Is Dependent on the Delivery of the T3E HopM1.

RISAP Accumulates Subapically Directly Behind the CZ Filled with Apical Vesicles in Normally Elongating Tobacco Pollen Tubes.(A) Single confocal optical.

LZY Genes Regulate Shoot Gravitropism in Statocytes.

Characterization of GmSIN1.

Comparison of Different Systems for Expressing Multiple gRNAs.

Bimolecular Fluorescence Complementation Assay Showing That BAM1 and LSF1 Interact in the Chloroplast. Bimolecular Fluorescence Complementation Assay Showing.

Kros of Single grf Knockout Mutants.

Clade 2 ERFs Differentially Bind and Activate a PMT2 Promoter

Circadian Rhythms of Kros and AQP Phosphorylation.

Presentation transcript:

Both AtSYP121-Sp2 and ZmSYP121-Sp2 Fragments Selectively Reduce the Accumulation of Zm-PIP2;5 in the Plasma Membrane of Maize Mesophyll Protoplasts.(A) Plasma membrane abundance of mYFP:ZmPIP2;5 in maize mesophyll protoplasts. Both AtSYP121-Sp2 and ZmSYP121-Sp2 Fragments Selectively Reduce the Accumulation of Zm-PIP2;5 in the Plasma Membrane of Maize Mesophyll Protoplasts.(A) Plasma membrane abundance of mYFP:ZmPIP2;5 in maize mesophyll protoplasts. (a) to (c) Protoplast expressing mYFP:ZmPIP2;5 (a). (b) Channel showing the FM4-64 fluorescence and chlorophyll a autofluorescence. (c) Merged image. (d) to (f) Protoplast coexpressing mYFP:PIP2;5 (d) and mCFP:PMA2 (e). (f) Merged image. (g) to (i) Protoplast coexpressing mYFP:PIP2;5 (g) and mCFP:AtSYP121-Sp2 (h). (i) Merged image. Bars = 5 µm.(B) Quantification of mYFP:ZmPIP2;5 fluorescence intensity in the protoplast plasma membrane (PM). Acquisitions were performed with the same settings for all samples. Each bar represents the mean of five independent experiments (10 protoplasts per experiment). Error bars show the 95% confidence interval. Student’s t test, P < 0.05 was used to compare the data. Means that are significantly different (P < 0.05) are indicated by different letters. a.u., arbitrary units.(C) Plasma membrane abundance of Zm-PIP2;5 in maize mesophyll protoplasts. (a) and (b) Protoplast expressing mYFP:ZmPIP2;5 (b). (a) Chlorophyll a autofluorescence. (c) and (d) Protoplast coexpressing the soluble mCFP (c) and mYFP:ZmPIP2;5 (d). (e) and (f) Protoplast coexpressing mCFP:ZmSYP121 (e) and mYFP:ZmPIP2;5 (f). (g) and (h) Protoplast coexpressing mCFP:ZmSYP121-Sp2 (g) and mYFP:ZmPIP2;5 (h). All the pictures were acquired with the same settings. In panel (a), the chloroplast autofluorescence is shown, whereas, in panels (c), (e), and (f), it is subtracted. Bars = 5µm.(D) Quantification of mYFP:ZmPIP2;5 fluorescence intensity in the protoplast plasma membrane. Each bar represents the mean of three independent experiments (10 protoplasts by experiments). Error bars show 95% confidence interval. Statistical differences were inferred by analysis of variance (ANOVA) followed by Tukey's honestly significant difference test. Means that are significantly different (P < 0.05) are indicated by different letters.(E) Plasma membrane abundance of mCFP:PMA2 in maize mesophyll protoplasts. (a) to (c) Protoplast expressing mCFP:PMA2 (a). (b) YFP channel. (c) Chlorophyll a channel. (d) to (f) Protoplast coexpressing mCFP:PMA2 (d) and mYFP:ZmSYP121-Sp2 (e). (f) Chlorophyll a channel. All the images were acquired with the same settings. Bars = 5 µm.(F) Quantification of mCFP:PMA2 fluorescence intensity in the protoplast plasma membrane. Each bar represents the mean of three independent experiments (10 protoplasts per experiment). Error bars show the 95% confidence interval. Means are not significantly different (Student’s t test, P = 0.90). Arnaud Besserer et al. Plant Cell 2012;24:3463-3481 ©2012 by American Society of Plant Biologists