1. Thermomorphogenesis in seedling organs is autonomous or interdependent.
Thermomorphogenesis in seedling organs is autonomous or interdependent. A, Temperature-induced elongation of hypocotyls, roots, and petioles (indicated by white arrows) in 8-d-old seedlings grown at 20°C or 28°C. B, Temperature-induced transcriptome responses in 6-d-old seedlings. Venn diagrams show overlap in differential gene expression after 24 h at 28°C versus control seedlings at 20°C (|log2 fold change [FC]| > 1, false discovery rate [fdr] < 0.01). C, Hierarchical clustering of DEGs using Euclidian distances of log2 FC data. D, MDS of DEGs (shown in C) based on the pairwise Pearson correlation (1 − cor) among all individual biological replicates. E, Elongation responses of detached seedling organs. Petioles and cotyledons or whole shoots were removed from 4-d-old seedlings grown at 20°C. Subsequently, detached organs were placed on growth medium and cultivated for an additional 4 d at 20°C or 28°C. Experiments were performed in long-day (16/8 h) conditions under 90 µmol m−2 s−1 white fluorescent light. Box plots show medians and interquartile ranges of total organ lengths; outliers (greater than 1.5× interquartile range) are shown as black dots. Different letters denote statistical differences at P < 0.05 as assessed by one-way ANOVA and Tukey’s honestly significant difference (HSD) posthoc test.
Julia Bellstaedt et al. Plant Physiol. 2019;180:
©2019 by American Society of Plant Biologists