1. dex-1 expression in the seam cells is regulated by DAF-16.
dex-1 expression in the seam cells is regulated by DAF-16. (A) We identified a putative DAF-16 IRS binding site (in capitals) ∼3 kb upstream from the dex-1 ATG start site. (B) Expression of dex-1p::gfp from a truncated 2.1 kb dex-1 promoter drives fluorescence in the seam cells (arrowheads) in both dauer (top) and nondauer (bottom) stages. Bar, 10 μm. (C) Dorsoventral view micrographs of GFP expression from the 5.7 kb dex-1 promoter in a wild-type background (top) produces bright fluorescence in the seam cells during dauer (also see Figure 5A). Fluorescence intensity is reduced in a daf-16(m27) partial dauer mutant background (middle). Deletion of the DAF-16 IRS sequence (bottom) from the 5.7 kb promoter region also significantly reduces GFP expression in the seam cells during dauer. Arrowheads indicate seam cells. Bar, 10 μm. (D) Quantification of GFP expression driven by the 5.7 kb dex-1 promoter in dauers. ** and *** indicate statistical significance at P < 0.01 and P < 0.001, respectively. Error bars indicate SEM.
Kristen M. Flatt et al. Genetics 2018;211:
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