1. Numerical analysis of cell morphology of tapetal cells in wild type.
Numerical analysis of cell morphology of tapetal cells in wild type. A, Z-projected images of PI-stained tapetal cells at different stages. Three-dimensional image stacks of cells were acquired by confocal microscopy and reconstructed by the software IMARIS. Scale bars = 5 μm. The PI micrographs were further used for numerical analysis of cell morphology, including volume (B) and shape (C and D). For quantification of cell morphology, length of axes of the cell was calculated. The y-, x-, and z-axes are the major, first minor, and second minor axes, respectively. B to D, Morphological changes of cell morphology of wild-type cells at different stages. Cell number quantified at each stage per experiment was ≥19. Three biological repeats were performed (n = 3). Results are shown as mean ± se. Statistically significant difference (*P < 0.05; **P < 0.01; ***P < 0.001) is analyzed by Student’s t test.
Pei-Ying Chen et al. Plant Physiol. 2019;180:
©2019 by American Society of Plant Biologists