Viperin−/−mice have an increased IFNγ Th1 response.

Slides:

Advertisements

Similar presentations

ContC1C2C3C4C5C6C7C8C9GCSE IgE (ng/ml) *** Supplement Fig 1. Hwang et al. Supplement Figure 1. Effect of GCSE and its.

Advertisements

SFig 1 sFig. 1. Apoptosis of UVB-irradiated NIT-1 cells. We treated NIT-1 cells by UVB- irradiation as described elsewhere. The cells were incubated in.

Reduced astrocyte proliferation results in increased immune cell infiltration into the injured GM Reduced astrocyte proliferation results in increased.

Anti-CD40 and CpG induce activation of T cells in draining lymph nodes

by Alexander Kiani, Francisco J

by Daniel L. Barber, Katrin D. Mayer-Barber, Lis R. V

Uptake of T-MPs for DC maturation and antigen presentation.

Wei Hu, Ty Dale Troutman, Ramakrishna Edukulla, Chandrashekhar Pasare

Volume 31, Issue 2, Pages (August 2009)

Interaction between B7-H1 and PD-1 determines initiation and reversal of T-cell anergy by Fumihiko Tsushima, Sheng Yao, Tahiro Shin, Andrew Flies, Sarah.

Langerin+ Dermal DC, but Not Langerhans Cells, Are Required for Effective CD8- Mediated Immune Responses after Skin Scarification with Vaccinia Virus

B-cell differentiation associates with down-regulation of vimentin expression. B-cell differentiation associates with down-regulation of vimentin expression.

Volume 137, Issue 3, Pages (September 2009)

IL-6 is dispensable for the suppressive activity of MDSC on primary CD4+ T-cell activation. IL-6 is dispensable for the suppressive activity of MDSC on.

Anti–4-1BB/PD-1 combination enhanced antigen-specific T-cell response.

Cytotoxic CD8+ T Cells Stimulate Hematopoietic Progenitors by Promoting Cytokine Release from Bone Marrow Mesenchymal Stromal Cells Christian M. Schürch,

DKO CD8+ T cells demonstrate a strong effector response but altered memory differentiation and maintenance after LCMV infection. DKO CD8+ T cells demonstrate.

Brian Yordy, Norifumi Iijima, Anita Huttner, David Leib, Akiko Iwasaki

MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific T-bet+ population. MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific.

Loss of pathogen-specific T cell memory is due to the absence of Runx2 in CD8+ T cells. Loss of pathogen-specific T cell memory is due to the absence of.

Protective Capacity of Memory CD8+ T Cells Is Dictated by Antigen Exposure History and Nature of the Infection Jeffrey C. Nolz, John T. Harty Immunity

Volume 21, Issue 3, Pages (September 2004)

Volume 32, Issue 2, Pages (February 2010)

Volume 25, Issue 4, Pages (April 2017)

Nonhematopoietic cells help regulate the pathogenic adaptive immune response during the second peak of joint swelling. Nonhematopoietic cells help regulate.

CD4 T cells are responsible for the intensified joint pathology at 6 d postinfection. CD4 T cells are responsible for the intensified joint pathology at.

CD4+ T Cells in Lymph Nodes of UVB-Irradiated Mice Suppress Immune Responses to New Antigens Both In Vitro and In Vivo Shelley Gorman, Jamie W.-Y. Tan,

Fig. 8 Combining M7824 with radiation or chemotherapy enhances antitumor efficacy. Combining M7824 with radiation or chemotherapy enhances antitumor efficacy.

Subcutaneous Infection with S

The psoriatic phenotype of DKO

IFN-γ induces TNF family ligand protein expression in vitro and in vivo. IFN-γ induces TNF family ligand protein expression in vitro and in vivo. (A and.

(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.

TNFα-producing CD3+CD4+CD44+ infiltrating cells.

Volume 13, Issue 2, Pages (February 2006)

Normal priming and expansion of functional T cells in the spleen during concurrent co‐infection Normal priming and expansion of functional T cells in the.

Molecular mechanism of STING-mediated inhibition of the mTORC1 pathway

Viperin−/− mice have high levels of pro-inflammatory cytokines but low levels of Th2 cytokines. Viperin−/− mice have high levels of pro-inflammatory cytokines.

Gating strategies for Fig 3.

BMDC-EV attract immune cells in lymph nodes similarly as in the skin.

Melissa B. Uccellini, Adolfo García-Sastre Cell Reports

T Cells with Low Avidity for a Tissue-Restricted Antigen Routinely Evade Central and Peripheral Tolerance and Cause Autoimmunity Dietmar Zehn, Michael.

Volume 24, Issue 1, Pages (January 2016)

Volume 8, Issue 2, Pages (August 2003)

CD44 Regulates Survival and Memory Development in Th1 Cells

“Responders” and “off-responders” proportions.

Figure 4 Increased susceptibility of MIF−/− CD4+ T cells to immunosuppression by Dex in EAE (A) MOG35-55 peptide-activated donor cells from wild-type (Wt)

IFN-γ–producing cells in the spleen and lungs postimmunization.

CD160 on CD8+ T cells is required for optimal clearance of L

Protective Regulatory T Cell Generation in Autoimmune Diabetes by DNA Covaccination with Islet Antigens and a Selective CTLA-4 Ligand Yelena Glinka,

Fig. 8 Combining M7824 with radiation or chemotherapy enhances antitumor efficacy. Combining M7824 with radiation or chemotherapy enhances antitumor efficacy.

Toll-Dependent Control Mechanisms of CD4 T Cell Activation

Volume 13, Issue 5, Pages (May 2013)

The number of islet antigen–specific T cells is reduced in CT-treated RIP-LCMV-GP mice. The number of islet antigen–specific T cells is reduced in CT-treated.

CD8 T cell memory alters the immune profile in enhanced HLH without altering viral load. CD8 T cell memory alters the immune profile in enhanced HLH without.

Figure 2 Effect of Dex on cytokine production by MIF−/− or Wt T cells in EAE Wild-type (Wt) and macrophage migration inhibitory factor (MIF)−/− mice were.

Interleukin-10-Treated Dendritic Cells Modulate Immune Responses of Naive and Sensitized T Cells In Vivo Gabriele Müller, Anke Müller Journal of Investigative.

BMS blocks functional responses in primary immune cells driven by IFNα

BLITC reporter for monitoring of GvHD.

Irradiated whole-cell vaccination with MOC1, but not MOC2, induces immunologic memory. Irradiated whole-cell vaccination with MOC1, but not MOC2, induces.

by Jianghong Zhong, Tatjana Scholz, Anthony C. Y

Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative. Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative.

MDSC-derived IL-6 enhances tumor progression through the inhibition of tumor-specific TH1 development and of their helper activity for CD8+ T cells. MDSC-derived.

Role of NO and IFNγ in mast cell–dependent MDSC-suppressive activities

Memory CD8+ T Cells Undergo Peripheral Tolerance

LSECtin, expressed by B16 cells, inhibits the tumor-specific immune responses both in vivo and in vitro. LSECtin, expressed by B16 cells, inhibits the.

Loss of polarity gene Dlg1 leads to an expansion of C'-1 stage cells during pre-B cell differentiation. Loss of polarity gene Dlg1 leads to an expansion.

Moderate-affinity vaccine antigens elicited greatest antitumor response. Moderate-affinity vaccine antigens elicited greatest antitumor response. Wild-type.

Varying the MHC-I affinity, TCR affinity or antigen dose alters the phenotype of CD8 T cells ex vivo. Varying the MHC-I affinity, TCR affinity or antigen.

Fig. 2 Cxxc1 deficiency restricts T cell–mediated autoimmunity and increases sensitivity to C. rodentium infection. Cxxc1 deficiency restricts T cell–mediated.

Design and operation of p.DOM-epitope vaccines.

Presentation transcript:

Viperin−/−mice have an increased IFNγ Th1 response. Viperin−/−mice have an increased IFNγ Th1 response. Animals were infected with 1 × 106 pfu CHIKV by footpad injection and monitored daily until 6 dpi when the draining lymph nodes were harvested and CD4 T cells were isolated. ELISpot was performed on 10,000 T cells isolated from individual mice with two sets of APCs from naive WT mice and two sets of APCs from naive Viperin−/− mice stimulated with CHIKV, E2EP3 peptide, or no antigen control. The stimulation ratio was calculated for each mouse as a mean of IFNγ spots from Viperin−/− APC stimulation divided by the mean of IFNγ spots from WT deficient APC stimulation. (A) IFNγ spot count for CD4 T cells stimulated by WT APCs and CHIKV. A representative ELISpot image is shown. (B) IFNγ spot count for the same WT CD4 T cells stimulated by different APC genotypes and CHIKV. A representative ELISpot image for the same T cells is shown. (C) IFNγ stimulation ratio during CHIKV stimulation calculated by dividing the spots induced by Viperin−/− APCs by the spots induced by WT APCs for the same T-cell from each individual mouse (spot counts are presented in Figs 4A and S7A. (D) IFNγ stimulation ratio during E2EP3 peptide stimulation (spot counts are presented in Fig S7B and C). The results are representative of three independent experiments for CHIKV stimulation (n = 13–14 mice per group), two independent experiments for E2EP3 stimulation (n = 8 mice per group), and were analyzed by Mann–Whitney nonparametric t test (**P < 0.01), and stimulation ratio was analyzed by one-sample t test to be different from 1 (***P < 0.001). Guillaume Carissimo et al. LSA 2019;2:e201900298 © 2019 Carissimo et al.