1. MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific T-bet+ population.
MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific T-bet+ population. (A) MP cells established in Rag γc KO mice do not contain a major Toxoplasma antigen AS15-specific population. Wild-type and Rag γc KO mice that had received CD4+ T cells 4 weeks earlier were infected with T. gondii or left uninfected, and 7 days later, CD4+ T lymphocytes from the PC and spleen of these animals were analyzed for CD44 expression and AS15 tetramer binding. The representative plots depict CD44 expression and AS15 tetramer binding for each group, whereas the bar graphs show the number (mean ± SD) of naïve, CD44hi, and CD44hi AS15 tetramer+ cells (n = 5). Data are representative of two independent experiments. (B) MP cells generated in Rag γc KO mice express T-bet. The histograms show T-bet expression by MP cells generated in Rag γc KO mice, as shown in (A). Filled histograms show negative control staining. Representative data from three animals were analyzed. (C) MP cells established in Rag γc KO mice do not produce IFN-γ in response to Toxoplasma antigen stimulation. Wild-type and Rag γc KO mice reconstituted with MP cells, as shown in (A), were infected with T. gondii, and on day 7 after infection, whole splenocytes and sorted CD44hi CD62Llo CD4+ T cells from the indicated groups were stimulated with medium, anti-CD3 mAb, STAg, or AS15 peptide in vitro. The graph indicates the concentration (mean ± SD) of IFN-γ in the culture supernatant from each group (n = 5). Data are representative of two independent experiments performed. *P < 0.05, **P < 0.01, ***P <
Takeshi Kawabe et al. Sci. Immunol. 2017;2:eaam9304
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