1. TNFα-producing CD3+CD4+CD44+ infiltrating cells.
TNFα-producing CD3+CD4+CD44+ infiltrating cells. Mice were infected with 1 × 106 pfu Fluc CHIKV by footpad injection and monitored daily until 6 dpi when the virus-infected joint footpad was harvested. Cells were stimulated for 4 h with PMA and ionomycin before staining for common cytokines. (A) Representative flow cytometry plot for CD44 and TNFα staining in WT and Viperin−/− cells with and without stimulation. (B) Median-centered proportion of baseline-subtracted proportion of TNFα T cells across three independent experiments (n = 13–14 per group). Gating strategies are presented in Fig S4, and the data were median-centered and analyzed by Mann–Whitney nonparametric t test.
Guillaume Carissimo et al. LSA 2019;2:e
© 2019 Carissimo et al.