Long-term results of tissue development and cell differentiation on dacron prostheses seeded with microvascular cells in dogs Gabriela Baitella-Eberle,

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Long-term results of tissue development and cell differentiation on dacron prostheses seeded with microvascular cells in dogs Gabriela Baitella-Eberle, PhD, Peter Groscurth, MD, Peter Zilla, MD, PhD, Mario Lachat, MD, Werner Müller-Glauser, PhD, Jakob Schneider, MD, Andrea Neudecker, MS, Ludwig K. von Segesser, MD, Eric Dardel, MD, PhD, Marko Turina, MD Journal of Vascular Surgery Volume 18, Issue 6, Pages 1019-1029 (December 1993) DOI: 10.1016/0741-5214(93)90557-3 Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Morphologic study of freshly isolated MVC. a-b, Appearance under light microscopy of endothelial clusters and bands (arrows), sheets of mesothelial cells (arrowheads), and rounded smooth muscle cells (double arrow). (Original magnification × 378.) c, TEM appearance of endothelial cells with typical junctional complexes (arrowheads) and Weibel-Palade bodies (arrows). (Original magnification × 4790.) d, Detail of mesothelial cell: note extremely flattened shape and low amount of cytoplasmic organelles. (Original magnification × 5800.) e, TEM appearance of rounded smooth muscle cells with perinuclearly located organelles and abundant actin filaments in cell periphery. (Original magnification × 7,880.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 SEM study of prostheses explanted 13 weeks after implantation. a-b, Seeded prosthesis: luminal surface is completely covered by elongated endothelial-like cells (a) displaying typical marginal flaps (b). (Original magnification: a, ×6610; b, ×3020.) c, Control prosthesis covered by mixed thrombi consisting of erythrocytes (arrow), platelets, and fibrin bundles. (Original magnification ×690.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Typical hematoxylin-eosin—stained histologic cross section of seeded Dacron prosthesis. Note well-organized, mature tissue layer. (Original magnification ×90.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 TEM morphologic study of endothelial cells in seeded grafts 5 weeks after implantation. Cells are highly differentiated with (a) basally condensed actin filaments (arrow), (b) Weibel-Palade bodies (arrowheads), and (b) numerous pinocytotic pits and vesicles at apical and basal portion. (Original magnification: a, ×4730; b, ×72,920.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Typical myofibroblasts found in subendothelial layer of seeded grafts 5 weeks after implantation. Note abundant profiles of rough endoplasmic reticulum and small rim of actin filaments in cell periphery. Myofibroblasts are embedded in well-defined extracellular matrix consisting of dense network of mature collagenous fibrils (inset). (Original magnification ×8,000; inset ×44,700.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 TEM study of subendothelial layer in seeded grafts obtained after 13 weeks of implantation. a, Myofibroblasts with characteristic features of smooth muscle cells. (Original magnification ×5540.) b, Attachment plaques (arrows) and basal lamina-like structure (arrowheads) at surface of myofibroblast. (Original magnification ×17,800.) c, Tannic acid staining of elastic fibers in extracellular matrix. (Original magnification ×11,100.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 7 TEM study of subendothelial layer in control grafts 26 weeks after implantation showing myofibroblast with typical features of a fibroblast. (Original magnification ×6900.) Journal of Vascular Surgery 1993 18, 1019-1029DOI: (10.1016/0741-5214(93)90557-3) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions