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Shunya Shindo, M. D. , Atsuhiko Takagi, M. D. , Anthony D

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Presentation on theme: "Shunya Shindo, M. D. , Atsuhiko Takagi, M. D. , Anthony D"— Presentation transcript:

1 Improved patency of collagen-impregnated grafts after in vitro autogenous endothelial cell seeding 
Shunya Shindo, M.D., Atsuhiko Takagi, M.D., Anthony D. Whittemore, M.D.  Journal of Vascular Surgery  Volume 6, Issue 4, Pages (October 1987) DOI: / (87) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

2 Fig. 1 Storage apparatus demonstrates Dacron graft supported between glass tubing struts through which endothelial cells may be seeded. Journal of Vascular Surgery 1987 6, DOI: ( / (87) ) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

3 Fig. 2 A, Scanning electron photomicrograph of collagen-coated Dacron graft (Weaveknit) shows endothelial cells spreading 3 days after seeding and before implantation. B, Scanning electron photomicrograph of collagen-coated graft (Microvel) 3 days after seeding and before implantation. (A and B, original magnification × 800.) Journal of Vascular Surgery 1987 6, DOI: ( / (87) ) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

4 Fig. 3 Scanning electron photomicrograph of midportion of a 12 cm long seeded Dacron graft, which had remained patent for 1 month in the canine femoral position. A continuous endothelial monolayer is demonstrated. (Original magnification × 800.) Journal of Vascular Surgery 1987 6, DOI: ( / (87) ) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

5 Fig. 4 A, Scanning electron photomicrograph of juxta-anastomotic region of nonseeded control collagen-coated Dacron graft shows marginal endothelial ingrowth. B, Scanning electron photomicrograph of midportion of the same graft shows lack of endothelium in the presence of fibrin and platelet deposition. (Original magnifications × 400 [A] and × 800 [B].) Journal of Vascular Surgery 1987 6, DOI: ( / (87) ) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

6 Fig. 5 Transmission electron photomicrograph of midportion of seeded Dacron graft after 1-month implantation shows an endothelial cell on the luminal surface overlying a smooth muscle cell. (Original magnification × 4500.) Journal of Vascular Surgery 1987 6, DOI: ( / (87) ) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

7 Fig. 6 A, Photomicrograph of seeded Dacron graft shows continuous endothelial monolayer overlying relatively thin subintima adjacent to the collagen coating. B, Photomicrograph of nonseeded control graft shows lack of endothelial monolayer on luminal surface and relatively thick subintima overlying sparse collagen coating. (Original magnifications, ×400 [A] and ×200 [B].) Journal of Vascular Surgery 1987 6, DOI: ( / (87) ) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions


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