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Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams,

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Presentation on theme: "Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams,"— Presentation transcript:

1 Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams, PhD, Leigh B. Kleinert, AS, Deborah Rose, BS, Sandra McKenney, BS, *  Journal of Vascular Surgery  Volume 19, Issue 4, Pages (April 1994) DOI: /S (94) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Immunofluorescent staining of falciform ligamental fat cryostat-cut sections after reaction with anti-vWF antibodies (a), with nuclear staining of same cryostat-cut section (b); or after reaction with anticytokeratin peptide 18 antibodies (c), with nuclear staining of same cryostat-cut section (d). Bar, 12 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Fluorescence emission from PKH-26–labeled, freshly isolated canine falciform ligamental cells plated on tissue culture plastic. Both attached (arrow) and round (arrowhead) cells emit fluorescence. Bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Growth curve of canine falciform ligamental cells. Cells were labeled with PKH-26 at a final dye concentration of 2 μmol/L (○), 5 μmol/L (▵); or without fluorescent label (□). Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Fluorescent micrograph of fluorescently labeled sodded graft before implantation. a, En face observation of the flow surface; bar, 36 mm. b, Cross section of the sodded graft; bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Morphologic characteristics of the PKH-26 labeled sodded grafts at the time of explantation. a, Scanning-electron micrograph of the midgraft flow surface; bar, 15 μm. b, Trichrome-stained section of the midgraft region; bar, 28 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 6 Fluorescence emission from the flow surface of sodded grafts at the time of explantation. Surfaces were evaluated with a rhodamine filter set. a, En face view of the flow surface; bar, 62 μm. b, Cross section of the flow surface; bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 7 a, Cross section of a sodded graft stained with antibody to vWF. Fluorescein isothiocyanate excitation and emission filter block; bar, 36 μm. b, Cross section of a segment of sodded graft incubated with DiI-ac-LDL. Rhodamine excitation and emission filter block; bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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