Supraphysiological ARSA activity in LV.hARSA‐injected NHP

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Supraphysiological ARSA activity in LV.hARSA‐injected NHP Supraphysiological ARSA activity in LV.hARSA‐injected NHP ASchematic representation of ARSA activity in the brain of S1.1 and S2.2 NHP along the rostrocaudal axis (slices 2–9). Data are expressed as fold increase to ARSA activity detected in matched blocks of UT and LV.GFP‐injected NHP (physiological levels). Grading of colors ranges from light yellow (< 1.2‐fold) to dark green (≥ twofold).B, CEnzymatic activity measured in single brain slices of NHP of study group 1 (B) and study group 2 (C) (filled gray bars, injected hemisphere; striped gray bars, contralateral hemisphere) and in UT and LV.GFP‐injected NHP (white bars; physiological levels). Arrows on x‐axis indicate the injection sites; n = 2–12 tissue blocks/slice. Number of tissue blocks analyzed per animal: 51 (UT/LV.GFP), 98–122 (S1.1 and S2.2), and 12–22 (S1.2, S1.3, S2.2, S2.3). Two‐way ANOVA followed by Bonferroni's multiple comparison tests, *P < 0.05; **P < 0.01; ***P < 0.001 versus matched slices of UT/LV.GFP.DSummary of ARSA activity in the injected and contralateral hemispheres of NHP in study group 1 and study group 2. Number of tissue blocks analyzed were as follows: 51 (UT/LV.GFP), 105 (study group 1, injected hemisphere) and 52 (study group 1, contralateral hemisphere); 103 (study group 2, injected hemisphere) and 72 (study group 2, contralateral hemisphere). One‐way ANOVA and Tukey's multiple comparison tests, ***P < 0.001 versus UT/LV.GFP, §P < 0.05 versus study group 1.EARSA activity in the spinal cord of individual LV.GFP‐ (n = 4 blocks/animal, 2–3 replicates) and LV.hARSA‐injected NHP (n = 2–4 blocks/animal; n = 10 and n = 11 total blocks for study group 1 and study group 2, respectively). Graph on the right represents the summary of ARSA activity in each treatment group. One‐way ANOVA and Dunnet's multiple comparison test. *P < 0.05 versus pilot group.FARSA activity in the sciatic nerve of LV.GFP‐ (n = 2) and LV.hARSA‐injected NHP (n = 3). In (B–F), data are expressed as floating bars (line at mean).GARSA activity in the cerebrospinal fluid (CSF) of UT/LV.GFP (n = 3) and LV.hARSA‐injected NHP (n = 5). Data are expressed as mean ± SEM. Student's t‐test, *P = 0.035. Vasco Meneghini et al. EMBO Mol Med. 2016;8:489-510 © as stated in the article, figure or figure legend