1. System approaches to analyze the effects of Zc3h10 knockdown
System approaches to analyze the effects of Zc3h10 knockdown Western blot analysis to detect Zc3h10 in SILAC light and heavy C2C12 myoblasts expressing scramble or Zc3h10 shRNA, respectively.Volcano plot of 3,712 proteins detected by SILAC analysis. Dark blue and red dots indicate downregulated and upregulated proteins, respectively, in shZc3h10 compared to scramble C2C12 myoblasts (P < 0.001). Blue and orange dots indicate downregulated and upregulated proteins, respectively (P < 0.01). Light blue and yellow dots indicate downregulated and upregulated proteins, respectively (P < 0.05). n = 1. P‐values by means of significance B were calculated by Perseus software [50,52].Gene ontology analysis of significantly downregulated protein clusters (P < 0.05) in myoblasts with Zc3h10 knockdown. P‐values are indicated as EASE score: modified Fisher exact P‐value.SILAC Oxphos subunit protein expression levels fold change in C2C12 myoblasts with Zc3h10 knockdown relative to scramble. Student's t‐test vs. scramble. *P < n = 1.SILAC TCA cycle enzymes protein expression levels fold change in C2C12 myoblasts with Zc3h10 knockdown relative to scramble. Student's t‐test vs. scramble. n = 1.Data information: n indicates the number of biological replicates. P‐values were calculated from the indicated n independent experiments according to [44].
Matteo Audano et al. EMBO Rep. 2018;19:e45531
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