A novel wheat gliadin as a cause of exercise-induced anaphylaxis

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A novel wheat gliadin as a cause of exercise-induced anaphylaxis

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Presentation transcript:

A novel wheat gliadin as a cause of exercise-induced anaphylaxis Kati Palosuo, MD, Harri Alenius, PhD, Elina Varjonen, MD, Minna Koivuluhta, BSc, Jari Mikkola, MSc, Helena Keskinen, MD, Nisse Kalkkinen, PhD, Timo Reunala, MD Journal of Allergy and Clinical Immunology Volume 103, Issue 5, Pages 912-917 (May 1999) DOI: 10.1016/S0091-6749(99)70438-0 Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 1 SDS-PAGE and Coomassie-blue staining of the purified 65-kd and 40-kd wheat proteins. Lane 1, Molecular weight marker; lane 2, wheat protein extract (acetonitrile-soluble); lane 3, γ-like gliadin (65-kd protein); lane 4, wheat protein extract (mainly water-soluble); lane 5, α-gliadin (40-kd protein). Journal of Allergy and Clinical Immunology 1999 103, 912-917DOI: (10.1016/S0091-6749(99)70438-0) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 2 IgE antibody levels to the γ-like gliadin (A ) and α-gliadin (B ) in ELISA (absorbance units) in 18 patients with wheat-dependent, exercise-induced anaphylaxis and 54 control subjects. Journal of Allergy and Clinical Immunology 1999 103, 912-917DOI: (10.1016/S0091-6749(99)70438-0) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 3 Inhibition of IgE binding from pooled patient sera to solid-phase wheat proteins with the γ-like gliadin (closed circles ) and α-gliadin (open circles ). Journal of Allergy and Clinical Immunology 1999 103, 912-917DOI: (10.1016/S0091-6749(99)70438-0) Copyright © 1999 Mosby, Inc. Terms and Conditions