Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis Sughashini Murugesu, M.B.B.Chir.,

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Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis Sughashini Murugesu, M.B.B.Chir., Srdjan Saso, Ph.D., M.R.C.O.G., M.R.C.S., Benjamin P. Jones, M.R.C.O.G., Timothy Bracewell- Milnes, M.R.C.O.G., Thanos Athanasiou, M.D., Ph.D., M.B.A., F.E.C.T.S., F.R.C.S., Anastasia Mania, B.Sc., M.Sc., H.C.P.C., Paul Serhal, M.D., M.R.C.O.G., Jara Ben-Nagi, M.D., M.B.B.S., M.R.C.O.G. Fertility and Sterility Volume 108, Issue 3, Pages 468-482.e3 (September 2017) DOI: 10.1016/j.fertnstert.2017.06.029 Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Search approach and selection of the studies for the meta-analysis (PRISMA statement flow diagram). Fertility and Sterility 2017 108, 468-482.e3DOI: (10.1016/j.fertnstert.2017.06.029) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Comparison of overall pregnancy rate following ET between AOA treatment and non-AOA treatment patient groups (random effects model). Fertility and Sterility 2017 108, 468-482.e3DOI: (10.1016/j.fertnstert.2017.06.029) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Comparison of live-birth rate after ET between AOA treatment and non-AOA treatment patient groups (random effects model). Fertility and Sterility 2017 108, 468-482.e3DOI: (10.1016/j.fertnstert.2017.06.029) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Comparison of fertilization rate between AOA treatment and non-AOA treatment patient groups (random effects model). Fertility and Sterility 2017 108, 468-482.e3DOI: (10.1016/j.fertnstert.2017.06.029) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Funnel plot test showing an asymmetrical plot. Fertility and Sterility 2017 108, 468-482.e3DOI: (10.1016/j.fertnstert.2017.06.029) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 Risk of bias summary (all studies). Fertility and Sterility 2017 108, 468-482.e3DOI: (10.1016/j.fertnstert.2017.06.029) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions