1. Low versus atmospheric oxygen tension for embryo culture in assisted reproduction: a systematic review and meta-analysis 
Carolina O. Nastri, Ph.D., Beatrice N. Nóbrega, M.D., Danielle M. Teixeira, M.D., Jowanka Amorim, M.Sc., Lívia M.M. Diniz, M.D., Marina W.P. Barbosa, M.D., Vanessa S.I. Giorgi, M.Sc., Vicky N. Pileggi, M.Sc., Wellington P. Martins, Ph.D. 
Fertility and Sterility 
Volume 106, Issue 1, Pages e17 (July 2016)
DOI: /j.fertnstert
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Study flow chart for the three parts of the review: RCTs for the first and second part and observational studies for the third part of the review. Obs = observational studies; RCT = randomized controlled trial.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

3. Supplemental Figure 1
Forest plot for live birth/ongoing pregnancy per randomized woman for the review of clinical outcomes. Comparison of “LowO2 versus AtmO2 during all embryo culture.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 2
Forest plot for clinical pregnancy per randomized woman for the review of clinical outcomes. Comparison of “LowO2 versus AtmO2 during all embryo culture.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 3
Forest plot for miscarriage per clinical pregnancy for the review of clinical outcomes. Comparison of “LowO2 versus AtmO2 during all embryo culture.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 4
Forest plot for live birth/ongoing pregnancy per randomized woman for the review of clinical outcomes. Comparison of “LowO2 versus AtmO2 after Day 2.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

7. Supplemental Figure 5
Forest plot for clinical pregnancy per randomized woman for the review of clinical outcomes. Comparison of “LowO2 versus AtmO2 after Day 2.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

8. Supplemental Figure 6
Forest plot for miscarriage per clinical pregnancy for the review of clinical outcomes. Comparison of “LowO2 versus AtmO2 after Day 2.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

9. Supplemental Figure 7
Forest plot for fertilization per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 during all embryo culture.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

10. Supplemental Figure 8
Forest plot for cleavage per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 during all embryo culture.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

11. Supplemental Figure 9
Forest plot for top cleavage embryos per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 during all embryo culture.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

12. Supplemental Figure 10
Forest plot for blastocyst per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 before Day 3 followed by low O2 in both groups.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

13. Supplemental Figure 11
Forest plot for top blastocyst per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 before Day 3 followed by low O2 in both groups.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

14. Supplemental Figure 12
Forest plot for cleavage embryos per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 only after Day 2.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

15. Supplemental Figure 13
Forest plot for blastocyst per oocyte randomized for the review of laboratory outcomes. Comparison of “LowO2 versus AtmO2 only after Day 2.” A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

16. Supplemental Figure 14
Funnel plot analysis of studies evaluating the effectiveness of LowO2 embryo culture on (A) live birth/ongoing pregnancy and (B) clinical pregnancy showing asymmetry.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

17. Supplemental Figure 15
Cumulative meta-analyses of studies evaluating the effect of LowO2 embryo culture on (A) live birth/ongoing pregnancy and (B) clinical pregnancy showing a trend toward a decrease in the observed effect over time.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

18. Supplemental Figure 16
Forest plot for live birth/ongoing pregnancy per randomized woman considering only the studies using the same incubator in both groups and comparing LowO2 and AtmO2 during all embryo culture. A/B = selection bias (sequence generation and allocation concealment); C = performance bias; D = detection bias; E = attrition bias; F = reporting bias; G = other bias.
Fertility and Sterility  , e17DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions