Reticulocyte Detection

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Presentation transcript:

Reticulocyte Detection Sysmex XE-2100 Reticulocyte Detection

Reticulocyte Methodology semiconductor laser flow cytometry method. RET-SEARCH (II) (diluent) RET-SEARCH (II) dye (stain) passed through the laser flow cytometer for analysis. Counted using forward light scatter and side fluorescence.

Flow Cytometry Forward light scatter - size. Side fluorescence - nuclear content.

RETIC Channel Explained

Therefore the RETIC Scattergram …

RETIC “Differential” Retic RNA content decreases on maturation. Thus fluorescence decreases with maturation. The XE2100 resolves the differential stages by fluorescence intensity.