1. Sysmex XE-2100
NRBC Detection

2. NRBC Counts - Methodology
From semiconductor laser flow cytometry.
STROMATOLYSER-NR (diluent)
STROMATOLYSER-NR dye (stain)
Passed through the laser flow cytometer for analysis.
Counted using side fluorescence and forward light scatter.

3. Flow Cytometry Forward light scatter - cell size
Side fluorescence - RNA/DNA information (nucleus)

4. NRBC Channel Explained

5. Therefore the NRBC Scattergram…

6. Resolution of NRBC and WBC
Clear & reliable separation of NRBC and WBC
Wbc take upp more dye than NRBC - so higher fluorescence
Smaller volume of the NRBC nucleus gives reduced forward light scatter.

7. CORRECTING WBC COUNT

8. The NRBC Count Algorithm
NRBC% = NRBC# x 100
NRBC# + WBC#
(NRBC/ 100 WBC)
WBC#
NRBC#

9. The Corrected WBC CountEo Ba Mo Ne Ly
NRBC
NRBC

10. NRBC # Revised Algorithm
WBC region in WBC/Baso and Diff scattergram include NRBC if NRBC appear

11. WBC & DIFF channels: Correction for NRBC’s
Correct WBC
Minus
Correct WBC Differential
Minus

12. NRBC Flags Swiching Algorithm
NRBC-CH is measured
“NRBC present” flag is monitored
(WBC abnormal flag category)
Detection from “NRBC%”
NRBC-CH is not measured
“NRBC?” flag is monitored
(WBC suspect flag category)
Detection from DIFF scattergram