1. FLOWCYTOMETRY

2. What Is Flowcytometry?
Flow→ Fluid
Cyto→ Cell
Metry→ Measurement

3. It allows simultaneous multi-parameter analysis of single cells
- Cell Size
- Granularity
- Cell Surface and Intracellular Molecules
- Cell Proliferation & Apoptosis
- DNA Analysis

4. How Does Flowcytometry Work?
- Fluidics
- Optics
- Electronics

5. Fluidics
When the stained cell sample in suspension buffer is run through the cytometer, the tiny “stream” of fluid takes the cells past the laser light one cell at a time.

6. Optics
Lasers producing light at specific wavelength
Scatter

7. Forward Scatter = Size of The Particle

8. Optics

9. Side Scatter = Granularity of The Cell

11. For Surface and Intracellular molecules
Antibody
Lable

12. Fluorescent Compound or Fluorochrome
Labeling of the antibody
Fluorescent Compound or Fluorochrome

13. Labeling of the antibody
Light
Fluorochrome
Excitation
Emission

14. Fluorochromes
Surface and Intracellular Staining

15. Optics

16. Data Analysis

17. Good luck