Inflammatory marker analysis in psoriatic skin under topical phosphodiesterase 4 inhibitor treatment Lennart M. Roesner, PhD, Petra Kienlin, Gabriele.

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Presentation transcript:

Inflammatory marker analysis in psoriatic skin under topical phosphodiesterase 4 inhibitor treatment Lennart M. Roesner, PhD, Petra Kienlin, Gabriele Begemann, Oliver Dittrich-Breiholz, PhD, Thomas Werfel, MD Journal of Allergy and Clinical Immunology Volume 140, Issue 4, Pages 1184-1187.e8 (October 2017) DOI: 10.1016/j.jaci.2017.04.026 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Immunohistological analysis of skin biopsy specimens. Markers were analyzed as cells/mm or intensity as indicated. P values were calculated using the paired t test for Gaussian distributed data or the Wilcoxon signed rank test for non-Gaussian distributed data. *P < .05. Journal of Allergy and Clinical Immunology 2017 140, 1184-1187.e8DOI: (10.1016/j.jaci.2017.04.026) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Genes strongly upregulated and downregulated in psoriasis lesions as detected by microarray analyses. Fold changes of vehicle-treated psoriatic skin lesion (PSL) samples versus healthy skin samples, 0.5% roflumilast–treated, and 5% TAK-084–treated PSL samples, as indicated (n = 4). Only top 35 upregulated and downregulated markers (healthy vs vehicle-treated skin) are depicted. Only targets with known gene function and without y-chromosome–specific expression are shown. Journal of Allergy and Clinical Immunology 2017 140, 1184-1187.e8DOI: (10.1016/j.jaci.2017.04.026) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Immunohistological analysis of dermal and epidermal infiltrating cells. Markers in the dermal and epidermal compartment were analyzed separately as cells/mm. Ratio was calculated as (cells/mm) dermis/(cells/mm epidermis). P values were calculated using the paired t test for Gaussian distributed data or the Wilcoxon signed rank test for non-Gaussian distributed data. *P < .05. Journal of Allergy and Clinical Immunology 2017 140, 1184-1187.e8DOI: (10.1016/j.jaci.2017.04.026) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Genes strongly upregulated and downregulated in psoriasis lesions as detected by microarray analyses. Fold changes of vehicle-treated psoriatic skin lesion (PSL) samples versus healthy skin samples, 0.5% roflumilast–treated, and 5% TAK-084–treated PSL samples, as indicated (n = 4). Only top 250 upregulated and downregulated markers (healthy vs vehicle-treated skin) are depicted. Journal of Allergy and Clinical Immunology 2017 140, 1184-1187.e8DOI: (10.1016/j.jaci.2017.04.026) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Transcriptional regulation of the cytokines IL-1 to IL-38. Fold changes of vehicle-treated psoriatic lesional skin (PSL) samples versus healthy skin samples, 0.5% roflumilast–treated, and 5% TAK-084–treated PSL samples, as indicated (n = 4). Journal of Allergy and Clinical Immunology 2017 140, 1184-1187.e8DOI: (10.1016/j.jaci.2017.04.026) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 In total, 9 biological processes were defined as significantly changed by gene ontology software (WEB-based GEne SeT AnaLysis Toolkit). Hierarchies of biological processes are displayed from left to right; colors indicate affiliation of genes to biological processes. Adjusted P values as well as contributing genes are indicated. Journal of Allergy and Clinical Immunology 2017 140, 1184-1187.e8DOI: (10.1016/j.jaci.2017.04.026) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions