Presentation is loading. Please wait.

Presentation is loading. Please wait.

Streptococcus pyogenes–induced cutaneous lymphocyte antigen–positive T cell– dependent epidermal cell activation triggers TH17 responses in patients with.

Published byRiitta Majanlahti Modified over 5 years ago

Similar presentations

Presentation on theme: "Streptococcus pyogenes–induced cutaneous lymphocyte antigen–positive T cell– dependent epidermal cell activation triggers TH17 responses in patients with."— Presentation transcript:

1 Streptococcus pyogenes–induced cutaneous lymphocyte antigen–positive T cell– dependent epidermal cell activation triggers TH17 responses in patients with guttate psoriasis  Ester Ruiz-Romeu, BSc, Marta Ferran, MD, Marc Sagristà, MD, Julià Gómez, PhD, Ana Giménez-Arnau, MD, PhD, Krisztina Herszenyi, MD, Péter Hóllo, MD, PhD, Antonio Celada, MD, PhD, Ramon Pujol, MD, PhD, Luis F. Santamaria-Babí, PhD  Journal of Allergy and Clinical Immunology  Volume 138, Issue 2, Pages e6 (August 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 SE-activated cocultures of CLA+ T cells and epidermal cells from patients with GP produce high levels of psoriasis-associated cytokines. Cocultures of supernatants from 14 patients with GP and 6 healthy control (CTRL) samples were collected at day 5, and IL-17A (A), IL-17F (B), IFN-γ (C), IL- 6 (D), TNF-α (E), and IL-8 (F) concentrations were measured. Data are represented by box plots (minimum to maximum and median ± interquartile range). Cytokine production by cocultures stimulated with SE were compared with basal cytokine values by using the Mann-Whitney U test: #P < .05 and ##P < .01. SE-stimulated conditions were compared by using the Kruskal-Wallis ANOVA test, followed by Dunn multiple comparison: *P < .05, **P < .01, and ***P < .001. Cell types and SE presence (+) for each coculture condition are defined below the x-axis. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 TH17 response is increased in HLA-Cw6+ patients with GP with previous pharyngitis. A-F, Cytokine production by cohort 1 (C-1, n = 6; except for IL-8 CLA−/Epi/SE, n = 3) and cohort 2 (C-2, n = 5). Data are expressed as box plots (minimum to maximum and median ± interquartile range). The Mann-Whitney U test was used to compare cytokine production: *P < .05 and **P < .01. NS, Not significant. Cell types and SE presence (+) for each coculture condition are defined below the x-axis. Linear correlation between cohort 1 SE-activated CLA+/Epi production of IL-17A (G) or IFN-γ (H) with time elapsed since disease onset. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Supernatants from psoriatic cohort 1–derived CLA+/Epi cocultures trigger a higher expression of psoriasis-associated transcripts in keratinocytes. A, Gene induction capacity of SE-activated CLA+/CLA− coculture supernatants from cohort 1 (GP C-1; n = 6) is compared with that of cohort 2 (GP C-2; n = 5) and control subjects (CTRL; n = 5). The Mann-Whitney U test was used for median comparisons: *P < .05 and **P < .01. B, Correlation between IL-17A concentrations of 5-day supernatants from SE-activated CLA+/Epi cocultures and the mRNA levels induced in keratinocytes. FI, Fold increase. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Expression of the skin barrier–associated genes FLG and LOR is downregulated by SE-activated CLA+/Epi coculture supernatants from GP cohort 1 samples. A, mRNA fold increase (FI) expression levels in keratinocytes treated with SE-activated CLA+/CLA− coculture supernatants from GP cohort 1 (GP C-1; n = 6), GP cohort 2 (GP C-2; n = 4), or healthy control subjects (CTRL; n = 5) samples. The Mann-Whitney U test was used for median comparisons: *P < .05 and **P < .01. B, Linear correlation between IL-17A concentrations within SE-activated CLA+/Epi coculture supernatants and respective mRNA expression in keratinocytes. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E1 TH17-predominant response in CLA+ T cells from patients with GP cultured with autologous epidermal cells induced by SE. A and B, Wilcoxon paired test analysis (marked as arrows) comparing IL-17A, IL-17F, and IFN-γ production in culture supernatants. C, Detailed paired data of IL-17A and IFN-γ cytokine concentrations from SE-activated CLA−/Epi cocultures (n = 13) from GP samples expressed as dots for each subject. NS, Not significant. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E2 Pairwise comparison indicates higher production of IL-17A than IFN-γ in cohort 1 CLA+/Epi/SE cocultures. Wilcoxon paired test analysis (marked as arrows) compared IL-17A, IL-17F, and IFN-γ production in supernatants from CLA+/Epi/SE (A) and CLA−/Epi/SE (B) cocultures. NS, Not significant. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E3 IFN-γ from SE-activated CLA+/Epi cell cocultures does not present a significant correlation with upregulation of gene expression in keratinocytes. Correlation between the contents of IFN-γ in the GP-derived supernatants from CLA+/Epi cocultures (cohort 1, n = 6; cohort 2, n = 5) and their respective mRNA levels of induction/reduction in keratinocytes. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Streptococcus pyogenes–induced cutaneous lymphocyte antigen–positive T cell– dependent epidermal cell activation triggers TH17 responses in patients with."

Similar presentations

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
Filaggrin-null mutations are associated with increased maturation markers on Langerhans cells  Claire S. Leitch, MBChB (Hons), MMed Sci, Eenass Natafji,

Filaggrin-null mutations are associated with increased maturation markers on Langerhans cells  Claire S. Leitch, MBChB (Hons), MMed Sci, Eenass Natafji,
TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis  Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis  Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.
Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,
Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,
Food protein–induced enterocolitis syndromes with and without bloody stool have distinct clinicopathologic features  Hideaki Morita, MD, PhD, Hiroko Suzuki,

Food protein–induced enterocolitis syndromes with and without bloody stool have distinct clinicopathologic features  Hideaki Morita, MD, PhD, Hiroko Suzuki,
Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders  Annick A.J.M.

Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders  Annick A.J.M.
Human circulating group 2 innate lymphoid cells can express CD154 and promote IgE production  Laura Maggi, PhD, Gianni Montaini, BSc, Alessio Mazzoni,

Human circulating group 2 innate lymphoid cells can express CD154 and promote IgE production  Laura Maggi, PhD, Gianni Montaini, BSc, Alessio Mazzoni,
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A
Sytze de Roock, PhD, Arie J

Sytze de Roock, PhD, Arie J
Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,
Filaggrin-null mutations are associated with increased maturation markers on Langerhans cells  Claire S. Leitch, MBChB (Hons), MMed Sci, Eenass Natafji,

Filaggrin-null mutations are associated with increased maturation markers on Langerhans cells  Claire S. Leitch, MBChB (Hons), MMed Sci, Eenass Natafji,
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,

IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,
Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels  Miguel L. Stein, MD, Joyce M. Villanueva,

Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels  Miguel L. Stein, MD, Joyce M. Villanueva,
Inflammatory marker analysis in psoriatic skin under topical phosphodiesterase 4 inhibitor treatment  Lennart M. Roesner, PhD, Petra Kienlin, Gabriele.

Inflammatory marker analysis in psoriatic skin under topical phosphodiesterase 4 inhibitor treatment  Lennart M. Roesner, PhD, Petra Kienlin, Gabriele.

Similar presentations

Ads by Google