PGAM5 associates with and activates ASK1.

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PGAM5 associates with and activates ASK1. PGAM5 associates with and activates ASK1. (A) Endogenous interaction between PGAM5 and ASK1. ASK1 was immunoprecipitated (IP) from the 10,000× g pellet (P) and supernatant (S) fractions of B16 cells, and coimmunoprecipitated PGAM5 was detected by immunoblotting (IB). (B) PGAM5 activates ASK1. HA-ASK1 and/or PGAM5 were expressed in HEK293 cells. Cell lysates were subjected to IB. P-ASK antibody recognizes phosphorylation of human ASK1 Thr-838. (C) PGAM5 activates JNK and p38 but not ERK. PGAM5 were expressed in HEK293 cells. For a positive control for ERK activation, cells were treated with 1 mM H2O2 for 15 min (indicated as H). Cell lysates were subjected to IB. Kohsuke Takeda et al. PNAS 2009;106:30:12301-12305 ©2009 by National Academy of Sciences