1. Pi and FGF2 differentially activate the FGFR1-FRS2α-ERK pathway.
Pi and FGF2 differentially activate the FGFR1-FRS2α-ERK pathway. (A) Galnt3 mRNA expression under 1 or 5 mM Pi or 100 ng/mL FGF2 for 48 h. (B) Immunoblotting with phosphorylated FRS2α at tyrosine 196 or tyrosine 436 from the lysates of UMR106 cells under 1 or 5 mM Pi, 100 ng/mL FGF2, or 5 mM sulfate for 15 min. (C and D) The time course of ERK1/2 phosphorylation (C, Left) or FRS2α phosphorylation at Tyr196 (D, Left) by 100 ng/mL FGF2 or 5 mM Pi. The Right shows the phosphorylated ERK1/2 (C, Right) or phosphorylated FRS2α levels (D, Right). Data represent the mean ± SEM. (A) n = 3 per group; *P < 0.05 by ANOVA with a post hoc Tukey’s test. (B–D) Data are presented as a representative image.
Yuichi Takashi et al. PNAS 2019;116:23:
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