1. Alterations to TNFR1 signaling and complex formation in the absence of TRADD. (A–C) ERK, JNK, and IκB. traddWT/WT and traddKO/KO MEFs (A) and BM-Macs (B) were stimulated with 10 ng/ml murine TNFα (A and B) or 10 ng/ml human TNF (C) for the indicat...
Alterations to TNFR1 signaling and complex formation in the absence of TRADD. (A–C) ERK, JNK, and IκB. traddWT/WT and traddKO/KO MEFs (A) and BM-Macs (B) were stimulated with 10 ng/ml murine TNFα (A and B) or 10 ng/ml human TNF (C) for the indicated times, and ERK and JNK phosphorylation and IκB degradation were assessed by Western blotting. β-tubulin and actin were used as loading controls. (D) TNFR1 complex composition. traddWT/WT and traddKO/KO MEFs were stimulated with 10 ng/ml TNFα for the indicated times, and the TNFR1 complex was isolated by IP. TRAF2, RIP, and ubiquitinated RIP (Ub-RIP) within the TNFR1 complex were detected by Western blotting. Data shown are representative of at least three independent experiments.
Nien-Jung Chen et al. PNAS 2008;105:34:
©2008 by National Academy of Sciences