Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis Eva Gros, MSc, Susanne Petzold,

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Presentation transcript:

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis Eva Gros, MSc, Susanne Petzold, MSc, Laura Maintz, MD, Thomas Bieber, MD, PhD, Natalija Novak, MD Journal of Allergy and Clinical Immunology Volume 128, Issue 5, Pages 1015-1021 (November 2011) DOI: 10.1016/j.jaci.2011.05.043 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Flow cytometric analysis of cell-surface IFN-γRI and IFN-γRII expression on epidermal skin DCs (A), monocytes (B), and MoDCs (C) and cell-surface IL-4Rα expression on monocytes (D) and MoDCs (E) from control donors (CTR; gray circles) and patients with AD (blue squares) are shown as scatter plots. ∗P < .05; ∗∗P < .005. Journal of Allergy and Clinical Immunology 2011 128, 1015-1021DOI: (10.1016/j.jaci.2011.05.043) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Relative expression of IRF-1 mRNA after IFN-γ stimulation of MoDCs from control subjects (CTR; gray bars, n = 18) and patients with AD (blue bars, n = 11) is shown. ∗P < .05. Journal of Allergy and Clinical Immunology 2011 128, 1015-1021DOI: (10.1016/j.jaci.2011.05.043) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Relative expression of IP-10/CXCL10 mRNA after IFN-γ stimulation of MoDCs from control subjects (CTR; gray bars, n = 18) and patients with AD (blue bars, n = 11) is shown (A). IP-10/CXCL10 concentration in the cell-culture supernatant of IFN-γ–pulsed MoDCs (n = 11) was measured by means of ELISA (B). IP-10/CXCL10 concentration in the cell-culture supernatant of unstimulated MoDCs was less than the detection minimum. ∗P < .05. Journal of Allergy and Clinical Immunology 2011 128, 1015-1021DOI: (10.1016/j.jaci.2011.05.043) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Relative expression of IDO mRNA after IFN-γ stimulation of MoDCs from control subjects (CTR; gray bars, n = 18) and patients with AD (blue bars, n = 11) is shown (A). Flow cytometric analysis of intracellular IDO expression in IFN-γ–pulsed MoDCs of CTR subjects (top) and patients with AD (bottom) is shown as density blots (B). Representative blots of 6 (CTR) and 9 (AD) experiments are shown. HPLC analysis of MoDC cell culture supernatants quantifying tryptophan (trp) degradation into its metabolite kynurenine (kyn) in CTR donors (gray line, n = 4) and patients with AD (blue line, n = 9) is shown (C). ∗P < .05; ∗∗P < .005. Journal of Allergy and Clinical Immunology 2011 128, 1015-1021DOI: (10.1016/j.jaci.2011.05.043) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Epidermal skin DCs (n = 6, A), monocytes (n = 15 and 16, respectively; B), and MoDCs (n = 10, C) from CTR donors (gray bars) and patients with AD (blue bars) were treated with the indicated IFN-γ concentrations and analyzed for intracellular STAT1 and p-STAT1 levels by means of flow cytometry. Journal of Allergy and Clinical Immunology 2011 128, 1015-1021DOI: (10.1016/j.jaci.2011.05.043) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions