Presentation is loading. Please wait.

Presentation is loading. Please wait.

Neuropeptide signaling through neurokinin-1 and neurokinin-2 receptors augments antigen presentation by human dendritic cells  Junya Ohtake, PhD, Shun.

Published byHartono Sasmita Modified over 4 years ago

Similar presentations

Presentation on theme: "Neuropeptide signaling through neurokinin-1 and neurokinin-2 receptors augments antigen presentation by human dendritic cells  Junya Ohtake, PhD, Shun."— Presentation transcript:

1 Neuropeptide signaling through neurokinin-1 and neurokinin-2 receptors augments antigen presentation by human dendritic cells  Junya Ohtake, PhD, Shun Kaneumi, MS, Mishie Tanino, MD, PhD, Takuto Kishikawa, MS, Satoshi Terada, MS, Kentaro Sumida, PhD, Kazutaka Masuko, PhD, Yosuke Ohno, MD, PhD, Toshiyuki Kita, PhD, Sadahiro Iwabuchi, PhD, Toshiya Shinohara, MD, PhD, Yoshinori Tanino, MD, PhD, Tamiko Takemura, MD, PhD, Shinya Tanaka, MD, PhD, Hiroya Kobayashi, MD, PhD, Hidemitsu Kitamura, PhD  Journal of Allergy and Clinical Immunology  Volume 136, Issue 6, Pages (December 2015) DOI: /j.jaci Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A and B, NK1R and NK2R gene expression levels in human MoDCs generated from PBMCs (N = 4) were evaluated by RT-PCR after stimulation with IFN-α, IFN-β, IFN-γ, LPS, or poly I:C for 24 hours. C, MoDC NK1R and NK2R expression levels after stimulation with poly I:C in the presence of a STAT1 inhibitor. D, NK1R and NK2R expression on DCs after poly I:C stimulation. Surface expression levels of NK1R and NK2R on CD11c+ DCs were evaluated by flow cytometry. Representative profiles and mean and SD (N = 4) of the calculated normalized mean fluorescence intensity (ratio MFI = MFI/respective isotype control) are shown. E, Hematoxylin-eosin staining and immunostaining of CD68, CD163, HLA-DR, NK1R, and NK2R were performed on sections of normal lung and lesion areas from a representative normal person and a patient with asthma or HP. Scale bar is 200 μm for all panels. F, Dual-immunofluorescence staining of CD68 (red) and NK1R or NK2R (green) was performed for the section from a patient with asthma or HP. Scale bar is 20 μm for all panels. DMSO, Dimethyl sulfoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. ∗P < .05 and ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 A, DCs (N = 6) were treated with poly I:C in the presence of NK1R or NK2R antagonists for 24 hours. Surface expression levels of HLA-A/B/C, HLA-DR, CD80, CD86, CD40, and CCR7 on CD11c+ DCs from PBMCs were evaluated by flow cytometry. Representative profiles and mean and SD (N = 6) of the calculated ratio mean fluorescence intensity (ratio MFI = MFI/respective isotype control) are shown. B, White birch antigen-specific CD4+ T cells (N = 4) were induced by Bet-v1 peptide-pulsed MoDCs for 2 weeks. IFN-γ and IL-4 production was evaluated by flow cytometry. Bet-v1–specific TH cells were activated by peptide-pulsed MoDCs in the presence of an NK1R or NK2R antagonist for 24 hours. Antigen-specific TH cells were counted, and the mean and SD are shown. C, Bet-v1–specific CD4+ T cells were cocultured with antigen-pulsed MoDCs in the presence of NK1R or NK2R antagonist for 24 hours. IL-2, IL-4, IL-5, IL-13, IFN-γ, and IL-12 concentration in culture supernatants was evaluated by ELISA. Three experiments were performed independently. Mean and SD of the representative data are shown. DMSO, Dimethyl sulfoxide. ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E5 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E6 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Fig E7 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Neuropeptide signaling through neurokinin-1 and neurokinin-2 receptors augments antigen presentation by human dendritic cells  Junya Ohtake, PhD, Shun."

Similar presentations

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M
Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,
Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F

Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
Human slan (6-sulfo LacNAc) dendritic cells are inflammatory dermal dendritic cells in psoriasis and drive strong Th17/Th1 T-cell responses  Anja Hänsel,

Human slan (6-sulfo LacNAc) dendritic cells are inflammatory dermal dendritic cells in psoriasis and drive strong Th17/Th1 T-cell responses  Anja Hänsel,
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells  Marc A. Williams,

Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells  Marc A. Williams,
Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,
Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,

IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,
Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal- dominant gain-of-phosphorylation STAT1 mutations  Pamela P.W. Lee,

Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal- dominant gain-of-phosphorylation STAT1 mutations  Pamela P.W. Lee,
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3  Se Jin Park,

Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3  Se Jin Park,
Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses  Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses  Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Similar presentations

Ads by Google