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Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum  Lianghua Bin, MD, PhD, Michael G. Edwards,

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Presentation on theme: "Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum  Lianghua Bin, MD, PhD, Michael G. Edwards,"— Presentation transcript:

1 Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum  Lianghua Bin, MD, PhD, Michael G. Edwards, PhD, Ryan Heiser, PhD, Joanne E. Streib, BA, Brittany Richers, BS, Clifton F. Hall, MS, Donald Y.M. Leung, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 134, Issue 4, Pages (October 2014) DOI: /j.jaci Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Expression profiles of PBMCs from patients with ADEH+ are distinct from those from patients with ADEH− after HSV-1 exposure. A, Venn diagram of genes significantly different (FDR < 0.05, ANOVA) in RNA-seq study between HSV-1 treatments and ADEH types. B, PCA analyses of the 4 experimental groups. HSV-1–stimulated patients with ADEH+, Red triangles; HSV-1–stimulated patients with ADEH−, red squares; sham-treated patients with ADEH+, blue triangles; and sham-treated patients with ADEH−, blue squares. A centroid connecting all associated members was added to the 4 different experimental groups in the PCA plot. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Induction of IFN-α by HSV-1 is significantly decreased in patients with ADEH+. A and B, IFNA4 (Fig 2, A) and IFNA5 (Fig 2, B) mRNA levels were evaluated by using real-time qPCR in sham- and HSV-1–treated PBMCs from healthy subjects (n = 20), patients with ADEH− (n = 20), and patients with ADEH+ (n = 20). C, IFN-α protein levels were evaluated by means of ELISA in culture supernatants from HSV-1–stimulated PBMCs from healthy subjects (n = 20), patients with ADEH− (n = 20), and patients with ADEH+ (n = 20). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Induction of the type III interferon IL-29 by HSV-1 is significantly decreased in patients with ADEH+. A, IL29 mRNA levels were evaluated by using real-time qPCR in sham- and HSV-1–treated PBMCs from healthy subjects (n = 20), patients with ADEH− (n = 20), and patients with ADEH+ (n = 20). B, IL-29 protein levels in HSV-1–stimulated PBMCs from healthy subjects (n = 20), patients with ADEH− (n = 20), and patients with ADEH+ (n = 20) were evaluated by using ELISA. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Upstream regulators of type I and type III interferons were inhibited in patients with ADEH+. A, IPA analysis demonstrated that 5 IRFs are predicted to be inhibited in patients with ADEH+. Each IRF is connected with its target genes; dashed and solid connecting lines indicate an indirect or direct relationship with the master regulator respectively. The colors of the lines connecting IRFs and their corresponding targets indicate the predicted activation status of the IRF gene based on expression patterns of its target genes. B, Statistical parameters of 5 IRFs. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Expression of IRF3 and IRF7 is significantly decreased in HSV-1–stimulated PBMCs from patients with ADEH+. IRF3 (A) and IRF7 (B) mRNA levels were evaluated by using real-time qPCR. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E3 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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