Sphingosine pathway deregulation in endometriotic tissues

Slides:

Advertisements

Similar presentations

Investigation of gene expression profiles before and after embryonic genome activation and assessment of functional pathways at the human metaphase II.

Advertisements

The impact of luteal phase support on gene expression of extracellular matrix protein and adhesion molecules in the human endometrium during the window.

TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation Gabriela dos Santos Hidalgo, M.Sc.,

David Langoi, D. V. M. , Mary Ellen Pavone, M. D. , M. S. C. I

Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls Hong Zhang,

Augmented epithelial multidrug resistance–associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A4 Ilaria Gori, Ph.D., Yoima.

Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium Kathleen M. Eyster, Ph.D., Olga Klinkova,

Daniel T. Rein, M. D. , Torsten Schmidt, M. D. , Gerd Bauerschmitz, M

Increased expression of c-fos protein associated with increased matrix metalloproteinase-9 protein expression in the endometrium of endometriotic patients

Risks of tubo-ovarian abscess in cases of endometrioma and assisted reproductive technologies are both under- and overreported Claire Villette, M.D.,

Increased expression of macrophage colony–stimulating factor and its receptor in patients with endometriosis Nicole M. Budrys, M.D., M.P.H., Hareesh.

Thyroid-stimulating hormone receptor and thyroid hormone receptors are involved in human endometrial physiology Lusine Aghajanova, M.D., Ph.D., Anneli.

Christine Jablonski, M. D. , Marco Alifano, M. D

Lymph node involvement and lymphovascular invasion in deep infiltrating rectosigmoid endometriosis Jean-Christophe Noël, M.D., Charles Chapron, M.D.,

Sébastien Colette, Ph. D. , Sylvie Defrère, Ph. D

Selective blockade of prostaglandin E2 receptors EP2 and EP4 signaling inhibits proliferation of human endometriotic epithelial cells and stromal cells.

TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation Gabriela dos Santos Hidalgo, M.Sc.,

Expression of motility-related molecule Cdc42 in endometrial tissue in women with adenomyosis and ovarian endometriomata Gaia Goteri, M.D., Andrea Ciavattini,

Increased messenger RNA expression of vascular endothelial growth factor and its receptors in the implantation site of the human oviduct with ectopic.

The expression of estrogen receptors as well as GREB1, c-MYC, and cyclin D1, estrogen-regulated genes implicated in proliferation, is increased in peritoneal.

Expression of microtubule associated protein 2 and synaptophysin in endometrium: high levels in deep infiltrating endometriosis lesions Martina Gori,

Aldo Cavallini, B. Sc. , Leonardo Resta, M. D

Increased expression of p21-activated kinase in adenomyosis

Regine Gaetje, M. D. , Uwe Holtrich, Ph. D. , Thomas Karn, Ph. D

Endometrial stromal progesterone receptor-A/progesterone receptor-B ratio: no difference between women with and without endometriosis Davide Gentilini,

Prospective assessment of midsecretory endometrial leukemia inhibitor factor expression versus ανβ3 testing in women with unexplained infertility Jason.

Growth differentiation factor 3 is induced by bone morphogenetic protein 6 (BMP-6) and BMP-7 and increases luteinizing hormone receptor messenger RNA.

Vascular density and distribution of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) are significantly higher in patients with.

Cell plating density alters the ratio of estrogenic to progestagenic enzyme gene expression in cultured granulosa cells Valério M. Portela, D.V.M., Ph.D.,

Two interesting cases of ovarian pregnancy after in vitro fertilization–embryo transfer and its successful laparoscopic management Selvaraj Priya, M.D.,

Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality Xingqi Zhang, Ph.D., Nadereh Jafari, Ph.D.,

Immunohistochemical detection of aquaporin expression in eutopic and ectopic endometria from women with endometriomas Xiu-Xiu Jiang, M.D., Riu-Jin Wu,

Lusine Aghajanova, M. D. , Ph. D. , Signe Altmäe, Ph. D

Xiao-Yu Pan, Ph. D. , Xue Li, M. D. , Zhan-Ping Weng, Ph. D

Endometrial LGR7 expression during menstrual cycle

Immunohistochemical detection of heparanase-1 expression in eutopic and ectopic endometrium from women with endometriosis Xiulong Xu, Ph.D., Jianchi.

Up-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic.

Prokineticin 1, homeobox A10, and progesterone receptor messenger ribonucleic acid expression in primary cultures of endometrial stromal cells isolated.

Deoxyribonucleic acid methyltransferases and methyl-CpG-binding domain proteins in human endometrium and endometriosis Kim J.A.F. van Kaam, M.D., Bert.

Murat Ulukus, M. D. , E. Cagnur Ulukus, M. D. , Ege N

Ching-wen Cheng, Ph. D. , Stephen K. Smith, M. D. , D

Lusine Aghajanova, M. D. , Ph. D. , Signe Altmäe, M. Sc

Christophe Poncelet, M. D. , Ph. D. , Françoise Cornelis, M. D

Ana Luiza L. Rocha, M. D. , Patrizia Carrarelli, B. Sc

Pietro Santulli, M. D. , Bruno Borghese, M. D. , Ph. D

Erica E. Marsh, M. D. , Zhihong Lin, Ph. D. , Ping Yin, Ph. D

Bone morphogenetic protein-6 stimulates gene expression of follicle-stimulating hormone receptor, inhibin/activin β subunits, and anti-Müllerian hormone.

Fei Wang, M. D. , Ph. D. , Zeqing Wen, M. D. , Hui Li, Ph. D

Jui-Hung Chang, Ph. D. , Heng-Kien Au, M. D. , Wei-Chin Lee, M. S

Autophagy is upregulated in ovarian endometriosis: a possible interplay with p53 and heme oxygenase-1 Giulia Allavena, M.S., Patrizia Carrarelli, Ph.D.,

Clinical uses of anti-Müllerian hormone assays: pitfalls and promises

Identification of X chromosome copies by quantitative real-time polymerase chain reaction for population screening tests Ester S. Ramos, M.D., Ph.D.,

Human myometrium and leiomyomas express gonadotropin-releasing hormone 2 and gonadotropin-releasing hormone 2 receptor Jason D. Parker, D.O., Minnie.

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease Idhaliz Flores, Ph.D., Elizabeth Rivera,

Prokineticin 1 mRNA expression in the endometrium of healthy women and in the eutopic endometrium of women with endometriosis Federica Tiberi, B.S.,

The steroidogenic factor-1 protein is not expressed in various forms of endometriosis but is strongly present in ovarian cortical or medullary mesenchymatous.

Katharina Hancke, M. D. , Oliver Strauch, V. M. D

Elevated levels of gremlin-1 in eutopic endometrium and peripheral serum in patients with endometriosis Guihua Sha, Ph.D., Yan Zhang, M.D., Chengyan.

Expression of leucine-rich repeat-containing G-protein-coupled receptors in the human cyclic endometrium Claudia A. Krusche, Ph.D., Tina Kroll, Henning.

Cleophas M. Kyama, Ph. D. , Attila Mihalyi, Ph. D

Insulin growth factors may be implicated in human sperm capacitation

Differences in gene expression in the proliferative human endometrium

Association of endometriosis with body size and figure

Expression and possible implication of growth hormone–releasing hormone receptor splice variant 1 in endometriosis Li Fu, Ph.D., M.D., Yutaka Osuga,

Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis Donald P. Braun, Ph.D., Jianchi Ding,

Nerve fibers and menstrual cycle in peritoneal endometriosis

Soluble HLA-G in the peritoneal fluid of women with endometriosis

A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model Daniel Escorsim Machado, M.Sc.,

Patrizia Carrarelli, M. Sc. , Ana Luiza Lunardi Rocha, M. D

Presentation transcript:

Sphingosine pathway deregulation in endometriotic tissues Pietro Santulli, M.D., Louis Marcellin, M.D., Jean-Christophe Noël, M.D., Ph.D., Bruno Borghese, M.D., Ph.D., Isabelle Fayt, M.D., Daniel Vaiman, Ph.D., Charles Chapron, M.D., Céline Méhats, Ph.D. Fertility and Sterility Volume 97, Issue 4, Pages 904-911.e5 (April 2012) DOI: 10.1016/j.fertnstert.2011.12.051 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Real-time polymerase chain reaction (PCR) gene expression analysis of sphingosine-1-phosphate metabolic enzymes in eutopic and ectopic endometrium of 16 different endometriotic women. Depicted is the relative amount of mRNA in endometriotic tissues vs. healthy endometrium. (Left) Baseline levels in endometrium of healthy women. Relative abundance: ++++ = detection between 18 and 25 cycles, very high expression; +++ = 26–28 cycles, high expression; ++ = 29–35 cycles, middle expression; + = 36–37 cycles, low expression; ± = 38–40 cycles, very low expression. The relative expression level was normalized using the geometric mean of three housekeeping genes. Values are mean. Statistical significance: ∗∗P<.01; ∗∗∗P<.001; ∗∗∗∗P<.0001. Fertility and Sterility 2012 97, 904-911.e5DOI: (10.1016/j.fertnstert.2011.12.051) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Real-time polymerase chain reaction (PCR) gene expression analysis of the five sphingosine-1-phosphate receptors in eutopic and ectopic endometrium from 16 different endometriotic women. Depicted is the relative amount of messenger RNA in endometriotic tissues versus healthy endometrium. (Left) Baseline levels in endometrium of healthy women. Relative abundance: ++++ = detection between 18 and 25 cycles, very high expression; +++ = 26–28 cycles, high expression; ++ = 29–35 cycles, middle expression; + = 36–37 cycles, low expression; ± = 38–40 cycles, very low expression. The relative expression level was normalized using the geometric mean of three housekeeping genes. Values are mean. Statistical significance: ∗P<.05; ∗∗∗P<.001; ∗∗∗∗P<.0001. Fertility and Sterility 2012 97, 904-911.e5DOI: (10.1016/j.fertnstert.2011.12.051) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 The S1PR2 expression (secretory phase) in normal endometrium of women without endometriosis (A) and with endometriosis (B), and in deep rectovaginal endometriosis (C) and in ovarian endometriosis (D). Note that the protein expression is mainly observed in the cytoplasm of the glandular component. Fertility and Sterility 2012 97, 904-911.e5DOI: (10.1016/j.fertnstert.2011.12.051) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Simplified scheme of sphingosine-1-phosphate (S1P) metabolism and its modification at the messenger RNA expression levels in eutopic and ectopic endometriotic tissues compared with healthy endometrium; hypothetic model of S1P axis amplification. (A) Baseline levels of messenger RNA expression in healthy endometrium; (B) changes in eutopic endometrium from endometriotic women; (C) changes in ectopic endometrium of endometriotic women compared with healthy tissues. The sizes of the boxes reflect the messenger RNA levels proportion of the different components of the S1P axis. Fertility and Sterility 2012 97, 904-911.e5DOI: (10.1016/j.fertnstert.2011.12.051) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions