1. Investigation of gene expression profiles before and after embryonic genome activation and assessment of functional pathways at the human metaphase II oocyte and blastocyst stage 
Georgia Kakourou, Ph.D., Souraya Jaroudi, Ph.D., Pinar Tulay, M.Sc., Carleen Heath, M.Sc., Paul Serhal, M.D., Joyce C. Harper, Ph.D., Sioban B. SenGupta, Ph.D. 
Fertility and Sterility 
Volume 99, Issue 3, Pages e23 (March 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Expression levels of selected commonly used housekeeping genes in the human oocyte and blastocyst. **P<.01; ***P<.001.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Expression levels of genes found to be stably expressed in different species. Blue bracket: human; red bracket: mouse; green bracket: rabbit; orange bracket: cow. *P<.05; **P<.01; ***P<.001.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Expression levels of microRNA machinery genes in human oocytes and embryo blastocysts. *P<.05, **P<.01.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 1
Genes differentially expressed in the blastocyst compared with the oocyte sample (P<.05). Genes were identified using the Celera dataset and grouped per Panther biologic process category; x axis = number of genes; y axis = Panther biologic process.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 2
Subcategories of the protein metabolism and modification biological process. Most genes showing higher expression in the blastocyst are involved in protein biosynthesis. Most protein modification genes were down-regulated at the blastocyst stage; x axis = number of genes; y axis = Panther biologic process.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

7. Supplemental Figure 3
Subcategories of the nucleic acid metabolism biologic process. The majority of genes involved in mRNA transcription showed higher expression in the oocyte. Genes involved in pre-mRNA processing, mostly in mRNA splicing, showed higher expression at the blastocyst stage; x axis = number of genes; y axis = Panther biologic process.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

8. Supplemental Figure 4
Grouping of 131 nucleic acid-binding category genes (128 function hits) that were ≥10-fold up-regulated in the human blastocyst.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

9. Supplemental Figure 5
Grouping of 105 nucleic acid-binding genes (80 hits) that were ≥10-fold down-regulated in the blastocyst.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

10. Supplemental Figure 6
Expression of five selected genes (DICER1, TMSB10, GTF2H2, ACTB, and NR2C1), relative to RPS24 in human oocytes and blastocysts, detected by real-time polymerase chain reaction. The results are expressed as mean ± SD of 2−ΔCt values derived from replicate experiments from each of three human oocyte (solid bars) and three human blastocyst samples (striped bars). Statistically significant differences between the sample types are indicated: DICER1: P=.0257; TMSB10: P=.0001; GTF2H2: no significant difference; ACTB: P=.0002; NR2C1: P= *P<.05, ***P<.001.
Fertility and Sterility  , e23DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions