Introduction to Urinalysis Laboratory Procedures

Urinary System Designed to maintain a balance between fluid, electrolytes, and acid-base homeostasis by selectively eliminating waste products from the body. Urine is formed through glomerular filtration, tubular reabsorption, and tubular secretion (remember everything I taught you in A&P). These are influenced by hormones Do you remember their names?

Medical Terminology Pollakiuria Polyuria Oliguria Anuria Dysuria Frequent urination Polyuria Increased urine output or production Oliguria Decrease in the formation or elimination of urine Anuria Complete absence of urine formation or elimination Dysuria Difficult urination

Urinalysis Urine collection can be accomplished through mid-stream free catch, manual expression, catheterization, and cystocentesis.

Advantages to Urinalysis Fast Simple Inexpensive Provides useful information (urinary tract and/or other body systems)

Voided Urine Sample Easiest to obtain May be contaminated from distal genital tract Not satisfactory if examining for bacteria.

Voided Urine Sample Collection Use a clean container Wasch prepuce or vulva (when possible) Try to collect mid-stream urine

Disadvantages to Voided Sample Contamination Difficult in cats May be difficult in easily scared dogs and short breeds

Expressing the Bladder Use steady gentle pressure Will feel like a balloon or ball under your hands. Make sure you are expressing in a squeezing motion where you are forcing urine down urethra. Wash external genitalia Contamination from lower urinary tract/genital tract is a concern. DO NOT DO MANUAL EXPRESSION WHEN THERE IS AN URETHRAL OBSTRUCTION!!!

Urinary Catheterization Act of placing a catheter through urethra into bladder Advantages: Less possibility of contamination from lower genital tract. Helpful in obese animals when bladder is difficult to palpate Disadvantages Trauma to sensitive urethral mucosa Possible contamination

Cystocentesis Act of obtaining a urine sample via a needle and syringe directly from the bladder Advantages Sterile sample Disadvantages Difficult to obtain in obese animals Trauma to organs if not done correctly

Urine Sample Preservation Analyze all urine within 30 minutes if possible May refrigerate for 6-12 hours if needed Bring to room temperature before anlaysis Morning samples are more concentrated If allowed to stand at room temperature, may get false results.

Physical Characteristics of Urine Color Transparency Odor Specific gravity Volume

Urine Color Normal color is due to pigments called urochromes Normal: light yellow to amber color Abnormals: Red: blood (hematuria) Reddish-brown: Hemoglobin or Myoglobin Dark yellow-brown: Bilirubin (bilirubinuria) Orange-Reddish brown: Normal in rabbits

Urine Transparency Clear vs. Cloudy Cloudy could indicate increase cells, mucus, casts, crystals and bacteria. Horses have cloudy urine due to high content of mucus and calcium carbonate crystals.

Urine Odor Not Very diagnostic Strong odor may suggesst bacterial urease production Male cats, goats, and pigs have a very strong urine odor

Urine Specific Gravity Measure urine concentration which is dependent on the number, molecular size, and weight of urine solutes Measures the density of urine as it compares to water Specific gravity of water is always 1.000

Normal Urine Specific Gravities Man: 1.015-1.035 Dog: 1.015-1.040 Cat: 1.015-1.050 Horse: 1.015-1.045

Methods of collecting a urine specific gravity Refractometer Reagent strip Urinometer

Causes of Altered Specific gravity Increased specific gravity Dehydration Increase fluid loss Decreased water intake Decreased specific gravity Kidney disease Excessive water Pyometra Some drugs

Urine Volume Influenced by several factors Water intake Size of animal Species of animal