Microbiology Practical I

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Presentation transcript:

Microbiology Practical I The following slides were contributed by your fellow BIO 251 students… Use side “” key to view slides…

Lab 1: Eukaryotes and Microscopy Know the parts of the microscope…

Lab 1: Eukaryotes and Microscopy Paramecium caudatum

Lab 1: Eukaryotes and Microscopy Euglena gracilis

Lab 1: Eukaryotes and Microscopy Amoeba proteus

Lab 1: Eukaryotes and Microscopy Fungus – Saccharomyces cerevisiae

Lab 1: Eukaryotes and Microscopy Cyanobacteria ~ Nostoc

~Bacteria slides~ Micrococcus luteus

~Bacteria Slides~ Bacillus subtilis

~Bacteria Slides~ Bacillus anthracis

~Bacteria Slides~ Rhodospirillum rubrum Sorry not in focus…

[ Adobe Acrobat of Gram and Endospore ] ~Bacteria Slides~ See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining… [ Adobe Acrobat of Gram and Endospore ] Some Endospore Staining ~

Streak Plate: Know colony characteristics (color, elevation, margin, etc.)

Spread Plate vs. Streak Plate:

Streak Plate:

Spread Plate: Mixed – Bacillus subtilis & Serratia marcescens

Practical I Unknown Tests: ~ Simple Stain ~ Gram Stain ~ Endospore Stain ~ Acid-Fast Stain (Demo) ~ Capsule Stain (Demo) ~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep) ~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)

Gas Pak Anaerobic vs. Aerobic:

Dilution Problems:

Dilution Problems:

Bacterial Growth Curve: Make sure you know how to: Use a spectrophotometer to measure absorbance. Produce a log graph of the calculations. Calculate generation time from a growth curve.