2. Cocci

3. Bacilli

4. Spirochetes

5. Staphylococcus Arrangement

6. Diplococcus

7. Streptococcus

8. Streptobacillus Arrangement

9. raised, Whole Colony MARGIN ELEVATION round irregular filamentous
rhizoid
MARGIN
Smooth,
entire
rhizoid
Irregular
(erose)
lobate
filamentous
ELEVATION
convex
umbonate
plateau
flat
raised,
spreading edge
flat raised
margin
growth into
medium
raised

10. Opaque Center and Spreading Edge

11. Shiny and Umbonate

12. Irregular and Rhizoid

13. Punctiform Colonies

14. Stains

16. Wet Mount (Hanging Drop)

17. Cheek Cells Without Stain

18. Cheek Cells with Simple Stain (Methylene Blue)

19. Simple Stain: Negative Stain

20. Negative Stain

21. Differential Stain: Gram Stain
Color of
Gram +
Gram –
Primary stain:
Crystal violet
Purple
Mordant:
Iodine
Decolorizing agent:
Alcohol-acetone
Colorless
Counterstain:
Safranin
Pink

22. Outer membrane
Peptidoglycan
GRAM NEGATIVE
GRAM POSITIVE

23. Gram Positive and Gram Negative Bacteria

24. Gram Stain of Staphylococcus epidermidis (+) and Citrobacter diversus (-).

25. Acid-Fast Stain ZN Method

26. Acid-Fast and Non Acid-Fast

27. Endospore Stains

28. Endospores

29. Endospore Stain: Ziehl-Neelsen

30. Figure 3.12a-c

31. Endospore Stain

32. Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST PRIMARY STAIN
ENDOSPORE
STAIN
PRIMARY STAIN
Crystal violet
Carbol
Fuschia
Malachite
green
MORDANT
Iodine
Heat
DECOLORIZER
ETOH or
acetone:
Cell is purple
Cell is clear
Acid
alcohol
Water
COUNTER-STAIN
Safranin:
Cell is pink
Methylene
Blue
Spores are

33. Capsule Stain

34. Capsule Stain

35. Figure 3.12a-c

36. Flagella Stain

37. Flagella Stain

38. Peritrichous Flagella

39. Figure 3.12a-c

40. Names for flagella arrangement
Monotrichous
Lophotrichous
Amphitrichous
Peritrichous