1. Bacillus sp
Basmah almaarik
#Lab 9

2. Bacillus culture Spore forming facultative anaerobic bacteria.
Grow every where cause it produce spores.
Grow in wide rage ºC
Spore formation is best seen in 25 – 30 ºC
Grow on NA and BAP

3. Species of bacillus Bacillus anthracis: Bacillus cereus:
Bacillus subtilis:
Causes anthrax; Mainly disease of sheep, cattle but human can be infected.
Highly infectious and pathogenic . Need special precaution to work with.
Produce toxin that cause food-poisoning. Toxin is produced when the bacilli sporulate, usually in rice that have been stored in warm temperatures.
Is not considered a human pathogen; it may contaminate food but rarely causes food poisoning

4. Culture of bacillus B. anthracis Aerobic
Large irregular colonies with wavy edges. (Ground glass appearance. )
Non haemolytic or slightly haemolytic
B. cereus
Aerobic
Large irregular colonies with wavy edges. (Ground glass appearance.)
Markedly haemolytic

5. Culture of bacillus
B. anthracis
B. cereus

6. All bacillus species are motile with the exception of B. anthracis.
Non motile.
Sensitive to Penicillin.
Capsulated
Motile
Resistant to penicillin.
Non-capsulated.
All bacillus species are motile with the exception of B. anthracis.
All bacillus species are generally resistance to penicillin with the exception of B. anthracis.
B. anthracis
B. cereus

7. Smear of bacillusus All Bacillus are Gram Positive Bacilli.
Have spores;
Inside or outside the cells
Central or terminal
B. anthracis
Non-motile.
Capsulated.
B. cereus
Motile.
Non-capsualated.

8. Spore stains Malachite Green (differential stain)
Nigrosin Stain (negative stain)
Ziehl-Neelsen Stianing

9. Malachite green stain Add drop of saline on a clean slide.
Touch organism with loop and emulsify in saline.
Spread, then fixe slide in hot plate.
Flood slide with warm malachite green for 5 m
Wash with water.
Counter stain with safranin 3-2 m
Wash then dray.

10. Spores can be inside or outside of cells. Central or terminal
Malachite green stain
Spores can be inside or outside of cells. Central or terminal

11. Nigrosin stain Add drop of saline on clean slide.
Touch organism by loop; emulsify in saline but do not spread.
Add 1 drop of nigrosin and 1 drop of safranin.
Mix all together by wooden stick.
Then spread by the edge of another slide.
Air dray and examin

12. Nigrosin stain Dark background Bacilli  colorless Spores  red
1 drop negrosin
Drop of saline with loopful of organism
Dark background
Bacilli  colorless
Spores  red
1 drop carbol fuschin
Mix al together then spread like blood film and examine.

13. Ziehl-neelsen stain
Back ground blue.
Bacilli  blue
Spores  red.